Transverse migration and microfluidic concentration of DNA using Newtonian buffers

We present experimental evidence that DNA can be concentrated due to an electrohydrodynamic coupling between a pressure-driven flow and a parallel electric field. The effects of buffer properties on the process were measured in a microfluidic channel. The concentration rates and the efficiency of tr...

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Bibliographic Details
Published inBiomicrofluidics Vol. 13; no. 4; p. 044104
Main Authors Montes, Ryan J., Ladd, Anthony J. C., Butler, Jason E.
Format Journal Article
LanguageEnglish
Published United States American Institute of Physics 01.07.2019
AIP Publishing LLC
Subjects
Buffer solutions
Deoxyribonucleic acid
DNA
Electric fields
Electrohydrodynamics
Microfluidics
Migration
Regular
Viscoelasticity
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Summary:We present experimental evidence that DNA can be concentrated due to an electrohydrodynamic coupling between a pressure-driven flow and a parallel electric field. The effects of buffer properties on the process were measured in a microfluidic channel. The concentration rates and the efficiency of trapping DNA were quantified as functions of the ion and polymer concentrations of the buffer solution. Buffers with large ion concentrations hindered the ability to trap DNA, reducing the short-time efficiency of the concentration process from nearly 100% to zero. Importantly, DNA was trapped in the microfluidic channel even when the buffer solution lacked any measurable viscoelastic response. These observations indicate that electrohydrodynamic migration drives the concentration of DNA. We found no evidence of viscoelastic migration in these experiments.
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butler@che.ufl.edu
ISSN:1932-1058
1932-1058
DOI:10.1063/1.5110718