Cell age distribution of erythrocytes at the incidence of cerebral stroke in stroke-prone spontaneously hypertensive rats, and their glutathione peroxidase activity

• Published in: Bioscience, biotechnology, and biochemistry Vol. 62; no. 3; pp. 484 - 490
• Main Authors: Murakami, T. (Kinki Univ., Higashiosaka, Osaka (Japan). Faculty of Agriculture), Takemori, K, Shirasaka, N, Yoshizumi, H, Ito, H
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.03.1998; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; AGE; Animals; Biological and medical sciences; Biotechnology; BRAIN; cell age distribution; CELLS; CELLULE; CELULAS; cerebral stroke; CEREBRO; Cerebrovascular Disorders - blood; Cerebrovascular Disorders - enzymology; EDAD; ENCEPHALE; ENZYMIC ACTIVITY; ERITROCITOS; ERYTHROCYTE; Erythrocyte Aging - physiology; ERYTHROCYTES; Erythrocytes - cytology; Erythrocytes - enzymology; GLUTATHION; GLUTATHIONE; glutathione peroxidase; Glutathione Peroxidase - blood; GLUTATION; HIPERTENSION; HYPERTENSION; Male; Medical sciences; Neurology; PEROXIDASAS; PEROXIDASES; PEROXYDASE; RAT; RATA; RATS; Rats, Inbred SHR; SHRSP; Vascular diseases and vascular malformations of the nervous system; Rat; Cardiovascular disease; Vascular disease; Enzymatic activity; Clinical biology; Cerebrovascular disease; Glutathione peroxidase; Hypertension; Nervous system diseases; Stroke; Enzyme; Ageing; Rodentia; Red blood cell; Antioxidant; Cerebral disorder; Feeding; Vertebrata; Mammalia; Diet therapy; Peroxidases; Animal; Central nervous system disease; Antihypertensive agent; Oxidoreductases
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.62.484

To study the mechanism of the fall of glutathione peroxidase (GSH-Px) activity in erythrocyte after cerebral strokes in stroke-prone spontaneously hypertensive rats (SHRSP), erythrocytes were fractionated into low density erythrocytes (LD-E) and high density erythrocytes (HD-E) by a density gradient centrifugal method using Percoll solution, and fluctuation of the distribution ratio and changes of GSH-Px activity in fractionated erythrocytes were investigated. The distribution ratio of LD-E and HD-E in erythrocytes of SHRSP was about 4:1 at 5 weeks of age (n=6), and the distribution to HD-E increased along with aging. While the distribution ratio was changed, however, there was no change in the GSH-Px activity in both LD-E and HD-E of erythrocytes. In senile, 30-week-old SHRSP (n=4) with advanced hypertension, the GSH-Px activity in the high was lower, in proportion to the increase of the distribution rate against HD-E. On the other hand, in SHRSP (n=5) having cerebral stroke, the distribution ratio of LD-E and HD-E was about 1:4. The GSH-Px activity was 31.4 +- 2.9 units/10(01) erythrocytes in LD-E, which was hardly different from the value of SHRSP without stroke. In HD-E, however, the activity was 18.2 +- 2.2 units/10(10) erythrocytes, being lower than the activity of SHRSP without stroke. At the moment when the GSH-Px activity had dropped to 17 units/mg hemoglobin, and the control diet was changed to one based on fish or a hydralazine treatment given, the activity recovered, and an increase in body weight and the distribution rate of the LD-E over HD-E was increased. It is clear from these experiments that the fall of erythrocyte GSH-Px activity observed after cerebral stroke is due to a decrease of LD-E and increase of HD-E, which has lowered activity. However, nothing definite is known on the relationship between the fall of GSH-Px activity in erythrocytes and disorder in cerebral tissue