No acute impact of haemodialysis treatment on free radical scavenging enzyme gene expression in white blood cells

• Published in: Journal of internal medicine Vol. 253; no. 2; pp. 201 - 207
• Main Authors: Schettler, V., Kühn, W., Kleinoeder, T., Armstrong, V. W., Oellerich, M., Müller, G. A., Wieland, E.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.02.2003; Blackwell Science; Blackwell Publishing Ltd
• Subjects: Aged; Biological and medical sciences; Diseases of the urinary system; Female; free radical scavenging enzymes; Free Radicals - metabolism; Gene Expression; haemodialysis; Humans; Leukocytes - enzymology; LightCycler; Male; Medical sciences; Oxidative Stress - physiology; Oxidoreductases - metabolism; oxygen radicals; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects); Renal Dialysis; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Messenger - metabolism; Superoxide Dismutase - metabolism; Superoxide Dismutase-1; white blood cells; Human; LightCycler® oxygen radicals; free radical scavenging enzymes; Immunopathology; Oxidative stress; Enzyme; Hemodialysis; Leukocyte; Quantization; haemodialysis; Exploration; Gene expression; Free radical; Extrarenal dialysis; Scavenging efficiency; Treatment; white blood cells; Molecular biology; Reverse transcription polymerase chain reaction
• ISSN: 0954-6820; 1365-2796
• DOI: 10.1046/j.1365-2796.2003.01088.x

.  Schettler V, Kühn W, Kleinoeder T, Armstrong VW, Oellerich M, Müller GA, Wieland E (Georg‐August‐University, Göttingen, Germany). No acute impact of haemodialysis treatment on free radical scavenging enzyme gene expression in white blood cells. J Intern Med 2003; 253: 201–207. Objective.  Oxidative stress has been implicated in the side‐effects caused by haemodialysis (HD) treatment. Design.  In the present study we have investigated whether gene expression of the enzymatic defence system provided by cellular glutathione peroxidase (GPx‐1), phospholipid glutathione peroxidase (GPx‐4), glutathione reductase (GSSG‐R), glutathione synthethase (GSH‐S), Cu/Zn‐superoxide dismutase (SOD‐1) and catalase (CAT) is affected by HD. The GPx‐1, GPx‐4, GSSG‐R, GSH‐S, SOD‐1 and CAT mRNA were determined in white blood cells by quantitative reverse transcriptase‐polymerase chain reaction with the LightCycler® instrument and transcription elongation factor‐2 as reference gene at the start (SD) and immediately after (ED) dialysis treatment (n = 36). In a subgroup (n = 10), messenger RNA (mRNA) expression was determined hourly during a 5 h HD. Results.  The expression of GPx‐1, GPx‐4, GSSG‐R, GSH‐S, SOD‐1 and CAT mRNA was not affected by a single HD treatment. All mRNAs were significantly (P < 0.05) increased in HD patients [median (16. percentiles (perc.); 84. perc.)]: GPx‐1: 2.18 (0.89; 3.23); GPx‐4: 0.41 (0.26; 0.74); GSSG‐R: 0.04 (0.02; 0.10); GSH‐S: 0.04 (0.02; 0.08); SOD‐1: 0.32 (0.20; 0.62); CAT: 0.12 (0.06; 0.18) when compared with healthy blood donors (GPx‐1: 0.91 (0.60; 1.44); GPx‐4: 0.27 (0.16; 0.43); GSSG‐R: 0.02 (0.01; 0.02); GSH‐S: 0.02 (0.02; 0.04); SOD‐1: 0.15 (0.10; 0.18); CAT: 0.07 (0.04; 0.16). Conclusions.  These results show that the HD procedure does not acutely affect the antioxidant defence system on the gene level but suggest that the chronic stress caused by uraemia and/or HD may cause gene induction of the enzymatic defence system.