Microfluidic transport in microdevices for rare cell capture

• Published in: Electrophoresis Vol. 33; no. 21; pp. 3133 - 3142
• Main Authors: Smith, James P., Barbati, Alexander C., Santana, Steven M., Gleghorn, Jason P., Kirby, Brian J.
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01.11.2012
• Subjects: Animals; Bioengineering; Cell Adhesion; Cell Separation - instrumentation; Cell Separation - methods; Circulating tumor cell; Humans; Mammals; Microfluidic Analytical Techniques - instrumentation; Microfluidic Analytical Techniques - methods; Microfluidic transport; Microfluidics; Models, Theoretical; Rare cell capture
• ISSN: 0173-0835; 1522-2683
• DOI: 10.1002/elps.201200263

The isolation and capture of rare cells is a problem uniquely suited to microfluidic devices, in which geometries on the cellular length scale can be engineered and a wide range of chemical functionalizations can be implemented. The performance of such devices is primarily affected by the chemical interaction between the cell and the capture surface and the mechanics of cell‐surface collision and adhesion. As rare cell‐capture technology has been summarized elsewhere (E. D. Pratt et al., Chem. Eng. Sci. 2011, 66, 1508–1522), this article focuses on the fundamental adhesion and transport mechanisms in rare cell‐capture microdevices, and explores modern device design strategies in a transport context. The biorheology and engineering parameters of cell adhesion are defined; adhesion models and reaction kinetics briefly reviewed. Transport at the microscale, including diffusion and steric interactions that result in cell motion across streamlines, is discussed. The review concludes by discussing design strategies with a focus on leveraging the underlying transport phenomena to maximize device performance.