Monoclonal anti-B as a new blood-typing reagent

Stable cloned lines of anti-B-secreting cells have been derived by fusing spleen cells of a mouse immunized by group B antigen with a mouse myeloma line. The tissue culture supernatant of one of them (NB1/19.112.28) containing secreted monoclonal anti-B antibody is an especially good blood-grouping...

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Bibliographic Details
Published inVox sanguinis Vol. 40; no. 2; p. 99
Main Authors Sacks, S H, Lennox, E S
Format Journal Article
LanguageEnglish
Published England 01.02.1981
Subjects
ABO Blood-Group System - immunology
Agglutination Tests
Animals
Antibodies
Antibodies, Monoclonal
Antibody Affinity
Antibody-Producing Cells - immunology
Blood Grouping and Crossmatching
Blood Preservation
Cells, Cultured
Humans
Hybrid Cells - immunology
Mice
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Summary:Stable cloned lines of anti-B-secreting cells have been derived by fusing spleen cells of a mouse immunized by group B antigen with a mouse myeloma line. The tissue culture supernatant of one of them (NB1/19.112.28) containing secreted monoclonal anti-B antibody is an especially good blood-grouping reagent, well suited for use with manual and automated methods. The unlimited availability of a potent reagent of uniform properties, cheaply produced by a single source, makes monoclonal anti-B a serious competitor to human typing serum.
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1981.tb00677.x