Diagnosis of human coronavirus infections in children using enzyme-linked immunosorbent assay

An enzyme-linked immunosorbent assay (ELISA) was developed for diagnosing human coronavirus (HCV) infections in children. One hundred and seventy seven nose swabs, throat swabs, and nasopharyngeal aspirates were collected from 30 children suffering from acute respiratory infections. These samples we...

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Bibliographic Details
Published inJournal of medical virology Vol. 11; no. 4; p. 319
Main Authors Macnaughton, M R, Flowers, D, Isaacs, D
Format Journal Article
LanguageEnglish
Published United States 1983
Subjects
Antigens, Viral - analysis
Child
Child, Preschool
Coronaviridae - immunology
Coronaviridae Infections - diagnosis
Enzyme-Linked Immunosorbent Assay
Humans
Immunoenzyme Techniques
Infant
Nasal Mucosa - immunology
Nasopharynx - immunology
Pharynx - immunology
Respiratory Tract Infections - diagnosis
Respiratory Tract Infections - etiology
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Summary:An enzyme-linked immunosorbent assay (ELISA) was developed for diagnosing human coronavirus (HCV) infections in children. One hundred and seventy seven nose swabs, throat swabs, and nasopharyngeal aspirates were collected from 30 children suffering from acute respiratory infections. These samples were tested for HCV antigens by ELISA and 28.2% of the samples were shown to be HCV positive. These results indicate that our ELISA should prove useful in the diagnosis of HCV infections in children. Further studies are in progress to extend the ELISA to detect HCVs in experimentally and naturally acquired infections in adults.
ISSN:0146-6615
DOI:10.1002/jmv.1890110407