Detection and identification of O-GlcNAcylated proteins by proteomic approaches

• Published in: Proteomics (Weinheim) Vol. 15; no. 5-6; pp. 1039 - 1050
• Main Authors: Vercoutter-Edouart, Anne-Sophie, Yazidi-Belkoura, Ikram El, Guinez, Céline, Baldini, Steffi, Leturcq, Maïté, Mortuaire, Marlène, Mir, Anne-Marie, Steenackers, Agata, Dehennaut, Vanessa, Pierce, Annick, Lefebvre, Tony
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01.03.2015; Wiley Subscription Services, Inc; Wiley-VCH Verlag
• Series: Proteomics
• Subjects: Acetylglucosamine - chemistry; Acetylglucosamine - metabolism; Animals; Cell Line; Chemical Sciences; Glycoproteins - analysis; Glycoproteins - chemistry; Glycoproteins - metabolism; Glycoproteomics; Glycosylation; Humans; Mass Spectrometry; Mice; O-GlcNAcome; O-GlcNAcylation; or physical chemistry; Post-translational modification; Protein Processing, Post-Translational; Proteomics - methods; Rats; Site mapping; Tandem Mass Spectrometry - methods; Theoretical and; Post-translational modification; O-GlcNAcylation; Glycoproteomics; Site mapping; O-GlcNAcome; Mass Spectrometry; Cell Line; Humans; Glycoprotein; Rats; Acetylglucosamine; Glycosylation; Post-Translational; Tandem Mass Spectrometry; Animals; Proteomics; Mice; Protein Processing
• ISSN: 1615-9853; 1615-9861
• DOI: 10.1002/pmic.201400326

O‐GlcNAcylation (O‐linked beta‐N‐acetylglucosaminylation) is a widespread PTM confined within the nuclear, the cytosolic, and the mitochondrial compartments of eukaryotes. Recently, O‐GlcNAcylation has been also detected in the close vicinity of plasma membranes particularly in lipid microdomains. The detection of this PTM can be easily done if appropriate controls and precautions are taken using a wide variety of tools including lectins, antibodies, or click‐chemistry‐based methods. In contrast, the identification of the proteins bearing O‐GlcNAc moieties and the localization of the precise sites of O‐GlcNAcylation remain challenging. This is due to the lability of the glycosidic bond between hydroxyl group of serine or threonine and N‐acetylglucosamine using conventional fragmentation techniques such as CID. To tentatively overcome this technical limitation, electron‐capture dissociation, or electron‐transfer dissociation MS/MS are now used. Thanks to these breakthroughs, a large number of O‐GlcNAc sites have been identified to date but these methodologies remain far from being used in routine.