The parAB gene products of Pseudomonas putida exhibit partition activity in both P. putida and Escherichia coli

• Published in: Molecular microbiology Vol. 43; no. 1; pp. 39 - 49
• Main Authors: Godfrin‐Estevenon, Anne‐Marie, Pasta, Franck, Lane, David
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.01.2002; Blackwell Publishing Ltd
• Subjects: Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Chromosomes, Bacterial - physiology; DNA, Bacterial; E coli; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Mutagenesis; parA protein; parB protein; Plasmids; Pseudomonas putida; Pseudomonas putida - genetics; Pseudomonas putida - growth & development; Pseudomonas putida - metabolism
• ISSN: 0950-382X; 1365-2958
• DOI: 10.1046/j.1365-2958.2002.02735.x

Summary The bacteria for which there is evidence that proteins of the ParAB family act in chromosome segregation also undergo developmental transitions that involve the ParAB homologues, raising the question of whether the partition activity is equivalent to that of plasmid partition systems. We have investigated the role in partition of the parAB locus of a free‐living bacterium, Pseudomonas putida, not known to pass through developmental phases. A parAB deletion mutant, compared with wild type, showed slightly higher frequencies of anucleate cells in exponen‐tially growing cultures but much higher frequencies in deceleration phase. This increase was growth medium dependent. Oversupply of ParA and ParB proteins also raised anucleate cell levels, specifically in the deceleration phase, in wild‐type and mutant strains and regardless of medium, as well as generating abnormal cell morphologies. Absence or oversupply of ParAB function had either slight or considerable effects on growth rate, depending on temperature and medium. The need for the Par proteins in chromosome partition thus appears to be subject to the cell’s physiological state. Three sequences similar to cis‐acting stabilization sites of Bacillus subtilis are present in the P. putida oriC–parAB region. One was inserted into an unstable mini‐F and shown to stabilize it in E. coli in a ParAB‐dependent manner.