Homing of a bacterial group II intron with an intron‐encoded protein lacking a recognizable endonuclease domain

• Published in: Molecular microbiology Vol. 35; no. 6; pp. 1405 - 1412
• Main Authors: Martínez‐Abarca, Francisco, García‐Rodríguez, Fernando M., Toro, Nicoláás
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.03.2000; Blackwell Publishing Ltd
• Subjects: Bacterial Proteins - genetics; Bacterial Proteins - metabolism; DNA Transposable Elements; DNA, Bacterial; Endonucleases - genetics; Endonucleases - metabolism; IEP protein; Introns; Plasmids - genetics; Rec A Recombinases - genetics; Rec A Recombinases - metabolism; Recombination, Genetic; Rhizobium - genetics; Sinorhizobium meliloti; Sinorhizobium meliloti - genetics; Zinc - metabolism
• ISSN: 0950-382X; 1365-2958
• DOI: 10.1046/j.1365-2958.2000.01804.x

RmInt1 is a functional group II intron found in Sinorhizobium meliloti where it interrupts a group of IS elements of the IS630‐Tc1 family. In contrast to many other group II introns, the intron‐encoded protein (IEP) of RmInt1 lacks the characteristic conserved part of the Zn domain associated with the IEP endonuclease activity. Nevertheless, in this study, we show that RmInt1 is capable of inserting into a vector containing the DNA spanning the RmInt1 target site from the genome of S. meliloti. Efficient homing was also observed in the absence of homologous recombination (RecA− strains). In addition, it is shown that RmInt1 is able to move to its target in a heterologous host (S. medicae). Homing of RmInt1 occurs very efficiently upon DNA target uptake (conjugation/electroporation) by the host cell resulting in a proportion of invaded target of 11–30%. Afterwards, the remaining intronless target DNA is protected from intron invasion.