Species assignment and antifungal susceptibilities of black aspergilli recovered from otomycosis cases in Iran

• Published in: Mycoses Vol. 55; no. 4; pp. 333 - 338
• Main Authors: Szigeti, Gyöngyi, Sedaghati, Ebrahim, Mahmoudabadi, Ali Zarei, Naseri, Ali, Kocsubé, Sándor, Vágvölgyi, Csaba, Varga, János
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.07.2012
• Subjects: Adult; Antifungal agents; Antifungal Agents - pharmacology; Aspergillosis - microbiology; Aspergillus niger; Aspergillus niger - classification; Aspergillus niger - drug effects; Aspergillus niger - isolation & purification; Calmodulin - genetics; Female; Fungal Proteins - genetics; Humans; Iran; Male; Microbial Sensitivity Tests; molecular typing; otomycosis; Otomycosis - microbiology; Phylogeny; Iran
• ISSN: 0933-7407; 1439-0507
• DOI: 10.1111/j.1439-0507.2011.02103.x

Summary Black aspergilli are among the main causative agents of otomycosis worldwide. In this study, the species assignment of black aspergilli isolated from otomycosis cases in Iran was carried out using sequence analysis of part of the calmodulin gene. The results indicate that Aspergillus niger is not the only black Aspergillus species involved in otomycosis cases in Iran: Aspergillus awamori and Aspergillus tubingensis are also able to cause ear infections. Antifungal susceptibility tests were carried out against five antifungal drugs including amphotericin B, fluconazole, itraconazole, ketoconazole and terbinafine. All isolates were highly susceptible to terbinafine, while they exhibited moderate susceptibilities against amphotericin B, fluconazole and ketoconazole. Aspergillus niger and A. awamori were found to have higher minimal inhibitory concentrations for azoles than A. tubingensis, in accordance with previous findings.