Rga5p is a specific Rho1p GTPase‐activating protein that regulates cell integrity in Schizosaccharomyces pombe
Summary Schizosaccharomyces pombe Rho1p regulates (1,3)β‐ d ‐glucan synthesis and is required for cell integrity maintenance and actin cytoskeleton organization, but nothing is known about the regulation of this protein. At least nine different S. pombe genes code for proteins predicted to act as Rh...
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Published in | Molecular microbiology Vol. 47; no. 2; pp. 507 - 518 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Science, Ltd
01.01.2003
Blackwell Publishing Ltd |
Subjects | |
Online Access | Get full text |
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Summary: | Summary
Schizosaccharomyces pombe
Rho1p regulates (1,3)β‐
d
‐glucan synthesis and is required for cell integrity maintenance and actin cytoskeleton organization, but nothing is known about the regulation of this protein. At least nine different
S. pombe
genes code for proteins predicted to act as Rho GTPase‐activating proteins (GAPs). The results shown in this paper demonstrate that the protein encoded by the gene named
rga5
+
is a GAP specific for Rho1p.
rga5
+
overexpression is lethal and causes morphological alterations similar to those reported for Rho1p inactivation.
rga5
+
deletion is not lethal and causes a mild general increase in cell wall biosynthesis and morphological alterations when cells are grown at 37°C. Upon mild overexpression, Rga5p localizes to growth areas and possesses both
in vivo
and
in vitro
GAP activity specific for Rho1p. Overexpression of
rho1
+
in
rga5Δ
cells is lethal, with a morphological phenotype resembling that of the overexpression of the constitutively active allele
rho1G15V.
In addition (1,3)β‐
d
‐glucan synthase activity, regulated by Rho1p, is increased in
rga5Δ
cells and decreased in
rga5
‐overexpressing cells. Moreover, the increase in (1,3)β‐
d
‐glucan synthase activity caused by
rho1
+
overexpression is considerably higher in
rga5Δ than in wild‐type cells. Genetic interactions suggest that Rga5p is also important for the regulation of the other known Rho1p effectors, Pck1p and Pck2p. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0950-382X 1365-2958 |
DOI: | 10.1046/j.1365-2958.2003.03312.x |