MICA Polymorphism Is Associated With Type 1 Diabetes in the Korean Population

• Published in: Diabetes care Vol. 24; no. 1; pp. 33 - 38
• Main Authors: Park, Y, Lee, H, Sanjeevi, C B, Eisenbarth, G S
• Format: Journal Article
• Language: English
• Published: Alexandria, VA American Diabetes Association 01.01.2001
• Subjects: Adolescent; Adult; Alleles; Analysis. Health state; Autoimmune diseases; Biological and medical sciences; Child; Child, Preschool; Chromosomes; Diabetes; Diabetes Mellitus, Type 1 - genetics; Diabetes. Impaired glucose tolerance; Disease; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Epidemiology; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Gene Frequency; General aspects; Genes; Genes, MHC Class I - genetics; Genes, MHC Class II - genetics; Genotype; Genotype & phenotype; Haplotypes; Humans; Korea; Linkage Disequilibrium; Medical sciences; Medicin och hälsovetenskap; Microsatellite Repeats; Middle Aged; Polymerase chain reaction; Polymorphism; Polymorphism, Genetic; Public health. Hygiene; Public health. Hygiene-occupational medicine; Research design; Software; Korea; Endocrinopathy; Human; Immunopathology; Short chain; HLA-DR-System; Chromosome 6; Korean; Autoimmune disease; Exon; Gene; Insulin dependent diabetes; Genetics; Caucasoid; Class I histocompatibility antigen; Polymorphism
• ISSN: 0149-5992; 1935-5548
• DOI: 10.2337/diacare.24.1.33

MICA Polymorphism Is Associated With Type 1 Diabetes in the Korean Population Yongsoo Park , MD , Hongkyu Lee , MD , Carani B. Sanjeevi , MD and George S. Eisenbarth , MD, PHD From the Department of Internal Medicine (Y.P., H.L.), Hanyang and Seoul National University, School of Medicine, Seoul, Korea; the Department of Molecular Medicine (C.B.S.), Karolinska Hospital, Stockholm, Sweden; and the Barbara Davis Center for Childhood Diabetes (G.S.E.), University of Colorado Health Sciences Center, Denver, Colorado. Address correspondence and reprint requests to Yongsoo Park, MD, Department of Internal Medicine, Hanyang University Hospital, 249-1 Kyomun-dong, Kuri, Kyunggi-do, 471-020, Korea. E-mail: parkys{at}email.hanyang.ac.kr . Abstract OBJECTIVE — Recent studies have demonstrated that MICA (major histocompatibility complex class I chain-related genes) on the short arm of the chromosome 6 are associated with susceptibility to various autoimmune diseases in Caucasians. The aim of our study was to investigate the role of MICA in type 1 diabetes susceptibility independent of the HLA DR-DQ polymorphism in genetically distinct Koreans. RESEARCH DESIGN AND METHODS — A total of 119 patients selected from Korean Seoul type 1 diabetes registry and 134 nondiabetic unrelated control subjects were typed for exon 5 polymorphism of MICA in addition to HLA DR-DQ typing. A total of 52 simplex families of type 1 diabetes were also studied. RESULTS — The MICA microsatellite allele consisting of six repetitions of GCT/AGC (A6) was present at a significantly lower frequency in the diabetic patient group ( P c < 0.01; P c = P value after Bonferroni correction) than in the control population. The MICA microsatellite allele consisting of four repetitions (A4) was present at a higher frequency in diabetic patients ( P < 0.05). This deviated distribution was not changed even after controlling for the HLA DRB1-DQB1 haplotype. Transmission/disequilibrium test revealed significant deviation of transmission for alleles at the A6 polymorphism within the MICA gene ( P < 0.05). CONCLUSIONS — We could assess that the MICA gene might be associated with type 1 diabetes transracially independent of the HLA gene. Footnotes Abbreviations: LD, linkage disequilibrium; MHC, major histocompatibility complex; MICA, MHC class I chain-related genes; PCR, polymerase chain reaction; RR, relative risk; TDT, transmission/disequilibrium test. A table elsewhere in this issue shows conventional and Système International (SI) units and conversion factors for many substances. Accepted September 21, 2000. Received June 13, 2000. by the American Diabetes Association, Inc.