A HPLC-Q-TOF-MS-based Urinary Metabolomic Approach to Identification of Potential Biomarkers of Metabolic Syndrome

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 34; no. 2; pp. 276 - 283
• Main Author: 于智睿 宁宇 于浩 汤乃军
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.04.2014; Tianjin Entry-Exit Inspection and Quarantine Bureau, Tianjin 300308, China%Department of Occupational and Environmental Health, Tianjin Medical University, Tianjin 300070, China; Department of Occupational and Environmental Health, Tianjin Medical University, Tianjin 300070, China; Offshore Oil Centers for Disease Control and Prevention, Tianjin 300452, China%Department of Occupational and Environmental Health, Tianjin Medical University, Tianjin 300070, China
• Subjects: 2型糖尿病; Adult; Biomarkers - urine; Chromatography, High Pressure Liquid; Female; Humans; Male; Mass Spectrometry; Medicine; Medicine & Public Health; Metabolic Syndrome - diagnosis; Metabolic Syndrome - urine; Metabolomics; Middle Aged; Principal Component Analysis; 代谢组学; 代谢综合征; 生物标志物; 芳香族氨基酸; 鉴定; 高效液相色谱; metabolic syndrome; HPLC-Q-TOF-MS; biomarker; metabolomics; urine
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-014-1271-7

Metabolic syndrome （MetS） is a serious threat to public health worldwide with an increased risk of developing type 2 diabetes, cardiovascular diseases and all-cause morbidity and mortality. In this study, a urinary metabolomic approach was performed on high performance liquid chromatography quadrupole time-of-flight mass spectrometry to discriminate 36 male MetS patients and 36 sex and age matched healthy controls. Pattern recognition analyses （principal component analysis and orthogonal projections to latent structures discriminate analysis） commonly demonstrated the difference between MetS patients and no-MetS subjects. This study found 8 metabolites that showed significant changes in patients with MetS, including branch-chain and aromatic amino acids （leucine, tyrosine, phenylalanine and tryptophan）, short-chain acylcanitine （tiglylcamitine）, tricarboxylic acid （TCA） cycle intermediate （cis-aconitic acid） and glucuronidated products （cortolone-3-glucuronide and tetrahydroaldoster- one-3-glucuronide）. The candidate biomarkers revealed in this study could be useful in providing clues for further research focusing on the in-depth investigation of the cause of and cure for MetS.