The Probiotic Combination of Lacticaseibacillus paracasei JY062 and Lactobacillus gasseri JM1 Alleviates Gastrointestinal Motility Disorder via Improving Gut Microbiota

• Published in: Nutrients Vol. 15; no. 4; p. 839
• Main Authors: Cheng, Shasha, Li, Hongxuan, Ding, Yixin, Huo, Jiacheng, Zheng, Yaping, Jiang, Yujun, Zhang, Yu, Man, Chaoxin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 07.02.2023; MDPI
• Subjects: Antibodies; Apoptosis; c-Kit protein; Cecum; Colon; Constipation; Drug dosages; Drug therapy; Feces; Gastric motility; Gastrin; gastrointestinal regulatory peptides; Gene expression; gut microbiota; Homeostasis; Interstitial cells; Interstitial cells of Cajal; Intestinal microflora; Intestine; KIT protein; Laboratory animals; Lacticaseibacillus paracasei; Lactobacillus gasseri; Metabolites; Microbiota; Moisture content; Motility; Nitric oxide; Nitric-oxide synthase; Peptides; Probiotics; Proteins; SCF/c-kit; Secretion; short-chain fatty acids; Small intestine; Vasoactive agents; Vasoactive intestinal peptide; Beijing China; United States > US; China; gastrointestinal regulatory peptides; SCF/c-kit; short-chain fatty acids; gut microbiota; probiotics
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu15040839

Probiotics have received wide attention as a potential way to alleviate gastrointestinal (GI) motility disorders. Herein, we investigated the effects of JY062, JM1, and the probiotic combination at 5 × 10 CFU/mL on mice induced by loperamide and explored the possible underlying mechanisms in GI motility disorder. After two weeks of probiotic intervention, the results indicated that the probiotic combination alleviated GI motility disorder better. It increased the secretion of excitatory GI regulators motilin, gastrin, and 5-hydroxytryptamine (5-HT) and decreased the secretion of the inhibitory GI regulators peptide YY and nitric oxide (NO), except vasoactive intestinal peptide. 5-HT and NO were related to the mRNA expression of 5-HT receptor and nitric oxide synthase, respectively. The intervention of probiotic combination also increased the number of interstitial cells of Cajal and the expression of SCF/c-kit protein. In addition, it also increased the abundance of beneficial bacteria ( , , and and improved the contents of short-chain fatty acids in cecum contents of mice. In conclusion, the probiotic combination of JY062 and JM1 has the potential to alleviate GI motility disorders by balancing intestinal homeostasis.