A novel cDNA, priBc, encoding a protein with a Zn(II)2Cys6 zinc cluster DNA-binding motif, derived from the basidiomycete Lentinus edodes

• Published in: Gene Vol. 139; no. 1; pp. 117 - 121
• Main Authors: Endo, Hirofumi, Kajiwara, Susumu, Tsunoka, Osamu, Shishido, Kazuo
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 11.02.1994; Amsterdam Elsevier; New York, NY
• Subjects: Amino Acid Sequence; amino acid sequences; Base Sequence; binding sites; Biological and medical sciences; biosynthesis; Cloning, Molecular; Cloning, Molecular - methods; complementary DNA; DNA-binding proteins; DNA-Binding Proteins - biosynthesis; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Escherichia coli; Escherichia coli Proteins; fruit bodies; fruiting; Fruiting body formation; Fundamental and applied biological sciences. Psychology; fungal anatomy; genbank/d01211; gene expression; Gene Library; Genes, Fungal; Genes, Regulator; Genes. Genome; genetics; Lentinula edodes; metabolism; methods; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; mushrooms; nucleotide sequences; Polyporaceae; Polyporaceae - genetics; pribc gene; recombinant DNA; Sequence Homology, Amino Acid; Southwestern blotting; structural genes; transcription factor; Zinc Fingers; Zinc Fingers - genetics; zinc-binding motif; aa; transcription factor; Fruiting body formation; SDS; nt; Southwestern blotting; PAGE; L; bp; mushrooms; tsp; kb; priBc; zinc-binding motif; recombinant DNA; Le; cDNA; PRIB; Fungi; Complementary DNA; Domain structure; Nucleotide sequence; DNA binding protein; Development; Basidiomycetes; Homology; Gene expression; Thallophyta; Lentinus edodes

A cDNA clone (designated priBc) was isolated from a primordial cDNA library of the basidiomycete, Lentinus edodes ( Le). The priBc clone consisted of 2628 bp encoding 565 amino acids. As was expected, the priB transcript was abundant in primordia, while preprimordial mycelia and mature fruiting bodi...