A novel cDNA, priBc, encoding a protein with a Zn(II)2Cys6 zinc cluster DNA-binding motif, derived from the basidiomycete Lentinus edodes

• Published in: Gene Vol. 139; no. 1; pp. 117 - 121
• Main Authors: Endo, Hirofumi, Kajiwara, Susumu, Tsunoka, Osamu, Shishido, Kazuo
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 11.02.1994; Amsterdam Elsevier; New York, NY
• Subjects: Amino Acid Sequence; amino acid sequences; Base Sequence; binding sites; Biological and medical sciences; biosynthesis; Cloning, Molecular; Cloning, Molecular - methods; complementary DNA; DNA-binding proteins; DNA-Binding Proteins - biosynthesis; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Escherichia coli; Escherichia coli Proteins; fruit bodies; fruiting; Fruiting body formation; Fundamental and applied biological sciences. Psychology; fungal anatomy; genbank/d01211; gene expression; Gene Library; Genes, Fungal; Genes, Regulator; Genes. Genome; genetics; Lentinula edodes; metabolism; methods; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; mushrooms; nucleotide sequences; Polyporaceae; Polyporaceae - genetics; pribc gene; recombinant DNA; Sequence Homology, Amino Acid; Southwestern blotting; structural genes; transcription factor; Zinc Fingers; Zinc Fingers - genetics; zinc-binding motif; aa; transcription factor; Fruiting body formation; SDS; nt; Southwestern blotting; PAGE; L; bp; mushrooms; tsp; kb; priBc; zinc-binding motif; recombinant DNA; Le; cDNA; PRIB; Fungi; Complementary DNA; Domain structure; Nucleotide sequence; DNA binding protein; Development; Basidiomycetes; Homology; Gene expression; Thallophyta; Lentinus edodes
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/0378-1119(94)90533-9

A cDNA clone (designated priBc) was isolated from a primordial cDNA library of the basidiomycete, Lentinus edodes ( Le). The priBc clone consisted of 2628 bp encoding 565 amino acids. As was expected, the priB transcript was abundant in primordia, while preprimordial mycelia and mature fruiting bodies contained lower levels of this Le transcript. The deduced PRIB protein (64kDa) contained a ‘Zn(II)2Cys6 zinc cluster’ DNA-binding motif. PRIB was produced in Escherichia coli using the bacteriophage T7 expression system. Southwestern blot analysis revealed that PRIB binds to the DNA fragment containing the upstream region of priB.