Comparison of the pro-inflammatory potential of monocytes from healthy adults and those with peripheral arterial disease using an in vitro culture model

• Published in: Atherosclerosis Vol. 193; no. 2; pp. 259 - 268
• Main Authors: Luu, N.T, Madden, J, Calder, P.C, Grimble, R.F, Shearman, C.P, Chan, T, Tull, S.P, Dastur, N, Rainger, G.E, Nash, G.B
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ireland Ltd 01.08.2007; Elsevier
• Subjects: Atherosclerosis; Atherosclerosis (general aspects, experimental research); Biological and medical sciences; Blood and lymphatic vessels; Cardiology. Vascular system; Cardiovascular; Cells, Cultured; Co-culture; Cytokines - biosynthesis; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous; Endothelial cells; Endothelial Cells - immunology; Humans; Leukocyte adhesion; Medical sciences; Monocytes; Monocytes - immunology; Neutrophil Activation; Neutrophil Infiltration; Peripheral arterial disease; Peripheral Vascular Diseases - immunology; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis; Tumor Necrosis Factor-alpha - immunology; Umbilical Veins; Peripheral arterial disease; Leukocyte adhesion; Monocytes; Co-culture; Endothelial cells; Atherosclerosis; Suboptimal donor; Cell culture; Endothelial cell; Leukocyte; Conservation; Cardiovascular disease; Hydrocortisone; Glucocorticoid; Arterial disease; Vascular disease; Human; Culture medium; Corticosteroid; Monocyte; Steroid hormone; Antiinflammatory agent; Adrenal hormone; Occlusive arterial disease; Neutrophil; Umbilical vein; Comparative study

Abstract We adapted a monocyte:endothelial cell co-culture model to investigate the pro-inflammatory potential of monocytes from patients with peripheral arterial disease (PAD). Isolated monocytes were cultured with human umbilical vein endothelial cells (HUVEC) for 24 h, after which the ability of...