Zein/gelatin/nanohydroxyapatite nanofibrous scaffolds are biocompatible and promote osteogenic differentiation of human periodontal ligament stem cells

• Published in: Biomaterials science Vol. 7; no. 5; pp. 1973 - 1983
• Main Authors: Ou, Qianmin, Miao, Yingling, Yang, Fanqiao, Lin, Xuefeng, Zhang, Li-Ming, Wang, Yan
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 23.04.2019
• Subjects: Biocompatibility; biocompatible materials; Biomedical materials; bone formation; Bones; Differentiation (biology); Gelatin; Human performance; humans; ligaments; Membranes; Nanofibers; Regeneration; Scaffolds; Stem cells; Tissue engineering; tissue repair; Wettability; Zein
• ISSN: 2047-4830; 2047-4849; 2047-4849
• DOI: 10.1039/c8bm01653d

In bone tissue engineering, it is important for biomaterials to promote the osteogenic differentiation of stem cells to achieve tissue regeneration. Therefore, it is critical to develop biomaterials with excellent cytocompatibility and osteoinductive ability. In our previous study, we found a zein/gelatin electrospinning scaffold with good biocompatibility, but low osteoinductive ability for human periodontal ligament stem cells (hPDLSCs). Therefore, herein, we fabricated novel zein/gelatin/nanohydroxyapatite (zein/gelatin/nHAp) nanofibrous membranes to overcome the drawbacks of the zein/gelatin scaffold. The results showed that the surface wettability of the zein/gelatin/nHAp nanofiber membranes was increased. Moreover, the inclusion of nHAp facilitated the attachment, proliferation, and osteogenic differentiation of hPDLSCs. Overall, the zein/gelatin/nHAp nanofiber membranes showed good biocompatibility and osteoinductive activity for hPDLSCs in vitro and in vivo ; this suggested potential applications of these membranes in bone tissue engineering. In bone tissue engineering, it is important for biomaterials to promote the osteogenic differentiation of stem cells to achieve tissue regeneration.