Cost-effective imprinting to minimize consumption of template in room-temperature ionic liquid for fast purification of chlorogenic acid from the extract of E. ulmoides leaves

• Published in: Analytical and bioanalytical chemistry Vol. 411; no. 6; pp. 1261 - 1271
• Main Authors: Sun, Ya Kun, Sun, Guang-Ying, Jia, Man, Yang, Jian, Liu, Zhao-Sheng, Huang, Yan-Ping, Aisa, Haji Akber
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.02.2019; Springer; Springer Nature B.V
• Subjects: ambient temperature; Analysis; Analytical Chemistry; Binding sites; Biochemistry; Characterization and Evaluation of Materials; Chemical synthesis; Chemistry; Chemistry and Materials Science; Chlorogenic acid; Chlorogenic Acid - isolation & purification; cost effectiveness; Crosslinking; Dimethyl sulfoxide; Esters; Ethylene glycol; Eucommia ulmoides; Eucommiaceae - chemistry; Flow velocity; Food Science; High flow; Identification and classification; Imidazoles - chemistry; Imprinted polymers; Ionic liquids; Ionic Liquids - chemistry; Laboratory Medicine; Leaves; Materia medica, Vegetable; Methods; Molecular Imprinting - economics; Molecular Imprinting - methods; Monitoring/Environmental Analysis; Morphology; Permeability; Plant extracts; Plant Leaves - chemistry; Polymerization; Polymers; Polymers - chemistry; Porosity; Properties; Purification; Pyridines - chemistry; Research Paper; Room temperature; Separation; Solid Phase Extraction - economics; Solid Phase Extraction - methods; Structure; China; Chlorogenic acid; Room-temperature ionic liquid; Molecularly imprinted polymer; Monolith; On-line solid-phase extraction
• ISSN: 1618-2642; 1618-2650; 1618-2650
• DOI: 10.1007/s00216-018-1559-8

One of the main challenges in large-scale applications of molecularly imprinted polymers (MIPs) is the significant amount of template needed in polymer preparation. A new strategy based on room-temperature ionic liquids (RTILs) was suggested to solve this problem by reducing the amount of template in the polymerization recipe. The MIP was synthesized with a mixture of dimethyl sulfoxide and RTIL (1-butyl-3-methylimidazolium tetrafluoroborate) as porogen, in which chlorogenic acid (CGA) was used as template, 4-vinylpyridine (4-VP) as functional monomer, and ethylene glycol dimethacrylate (EDMA) as cross-linker. The influence of polymerization variables, including CGA concentrations, and the ratio of 4-VP to EDMA on imprinting effect were investigated comprehensively. Moreover, the properties involving the column permeability, the number of binding sites, and the polymer morphology of the CGA-MIP monoliths were studied thoroughly. The MIP monolith had an excellent column permeability (1.53 × 10 −13  m 2 ) and allowed an ultra-fast on-line SPE, which dramatically shortens the separation time (< 10 min) and improves the separation efficiency. At high flow velocity (5.0 mL min −1 ), 50 μL of the extract from Eucommia ulmoides leaves can be loaded directly on the CGA-MIP monoliths and CGA with high purity can be obtained with a recovery of 89.01 ± 0.05%. As a conclusion, the resulting RTIL-induced approach of preparing MIP may be an effective tool in fabricating MIP in a low-cost way. Graphical abstract ᅟ