The human ASCL2 gene escaping genomic imprinting and its expression pattern

• Published in: Journal of assisted reproduction and genetics Vol. 19; no. 5; pp. 240 - 244
• Main Authors: MIYAMOTO, Toshinobu, HASUIKE, Shiga, JINNO, Yoshihiro, SOEJIMA, Hidenobu, YUN, Kankatsu, MIURA, Kiyonori, ISHIKAWA, Mutsuo, NIIKAWA, Norio
• Format: Journal Article
• Language: English
• Published: New York, NY Kluwer/Plenum 01.05.2002; Springer Nature B.V; Kluwer Academic Publishers-Plenum Publishers
• Subjects: Basic Helix-Loop-Helix Transcription Factors; Biological and medical sciences; Blotting, Northern; Chromosomes; Cloning; DNA - chemistry; DNA - genetics; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Embryology: invertebrates and vertebrates. Teratology; Embryonic and Fetal Development - genetics; Embryonic and Fetal Development - physiology; Female; Fetuses; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Developmental - genetics; Gene Expression Regulation, Developmental - physiology; Genes; Genomic Imprinting - genetics; Genomic Imprinting - physiology; Humans; Hybridization; In Situ Hybridization; Organogenesis. Fetal development; Organogenesis. Physiological fonctions; Placenta; Placenta - metabolism; Polymerase chain reaction; Polymorphism, Restriction Fragment Length; Pregnancy; Pregnancy Trimester, First; Pregnancy Trimester, Second; Random Amplified Polymorphic DNA Technique; Transcription Factors; United States > US; Human; Trophoblaste; Embryo; Placenta; Fetal membrane; Imprinting; Gene expression; Transcription factor
• ISSN: 1058-0468; 1573-7330
• DOI: 10.1023/a:1015362903486

The mouse achaete-scute homolog-2 gene (Ascl2 or Mash2) encodes a transcription factor playing a role in the development of the trophoblast. The Ascl2 is an imprinted gene with maternal expression and assigned to an imprinting gene cluster region (ICR) at a distal region of mouse chromosome 7. We previously isolated a phage clone carrying the human homolog, ASCL2, and mapped it to human chromosome 11p15.5, a human ICR. In the present study, we demonstrate the expression patterns of the human ASCL2 in the fetus at a stage between first and second trimesters and in the placental tissues. In addition, it has been shown that the human ASCL2 gene escapes genomic imprinting.