In vitro and in vivo studies of a novel nanohydroxyapatite/superhydrophilic vertically aligned carbon nanotube nanocomposites

• Published in: Journal of materials science. Materials in medicine Vol. 24; no. 7; pp. 1723 - 1732
• Main Authors: Lobo, Anderson Oliveira, Siqueira, Idalia A. W. B., das Neves, Marcele F., Marciano, Fernanda Roberta, Corat, Evaldo Jose, Corat, Marcus Alexandre F.
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.07.2013; Springer; Springer Nature B.V
• Subjects: Animals; Biological and medical sciences; Biomaterials; Biomedical Engineering and Bioengineering; Biomedical materials; Bones; Calcification, Physiologic - drug effects; Calcification, Physiologic - physiology; Cells, Cultured; Ceramics; Chemistry and Materials Science; Composites; Durapatite - chemistry; Durapatite - pharmacology; Glass; Guided Tissue Regeneration - instrumentation; Guided Tissue Regeneration - methods; Humans; Hydrophobic and Hydrophilic Interactions; Male; Materials Science; Materials Testing; Medical sciences; Mice; Mice, Inbred C57BL; Nanocomposites; Nanocomposites - chemistry; Nanotubes; Nanotubes, Carbon - chemistry; Natural Materials; Osteoblasts - cytology; Osteoblasts - drug effects; Osteoblasts - physiology; Polymer Sciences; Regenerative Medicine/Tissue Engineering; Surfaces and Interfaces; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Thin Films; Tissue engineering; Tissue Engineering - instrumentation; Tissue Engineering - methods; Bone Tissue; Simulated Body Fluid; Bone Tissue Regeneration; Bone Regeneration; Human Osteoblast Cell; In vivo; Hydroxyapatite; Nanocomposite; In vitro; Composite material; Biomedical engineering
• ISSN: 0957-4530; 1573-4838
• DOI: 10.1007/s10856-013-4929-y

An association between in vitro and in vivo studies has been demonstrated for the first time, using a novel nanohydroxyapatite/superhydrophilic vertically aligned multiwalled carbon nanotube (nHAp/VAMWCNT-O 2 ) nanocomposites. Human osteoblast cell culture and bone defects were used to evaluate the in vitro extracellular matrix (ECM) calcification process and bone regeneration, respectively. The in vitro ECM calcification process of nHAp/VAMWCNT-O 2 nanocomposites were investigated using alkaline phosphatase assay. The in vivo biomineralization studies were carried out on bone defects of C57BL/6/JUnib mice. Scanning electron microscopy, micro-energy dispersive spectroscopy, X-ray photoelectron spectroscopy, and X-ray difractometry analyses confirmed the presence of the nHAp crystals. nHAp/VAMWCNT-O 2 nanocomposites induced in vitro calcification of the ECM of human osteoblast cells in culture after only 24 h. Bone regeneration with lamellar bone formation after 9 weeks was found in the in vivo studies. Our findings make these new nanocomposites very attractive for application in bone tissue regeneration. Graphical Abstract