Whole Genome Sequence of an Edible Mushroom Oudemansiella raphanipes (Changgengu)

• Published in: Journal of fungi (Basel) Vol. 9; no. 2; p. 266
• Main Authors: Zhu, Liping, Gao, Xia, Zhang, Meihua, Hu, Chunhui, Yang, Wujie, Guo, Lizhong, Yang, Song, Yu, Hailong, Yu, Hao
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 16.02.2023; MDPI
• Subjects: Biodegradation; CAZyme; Comparative analysis; Enzymes; Genes; genome; Genomes; Mating; monokaryon; Monokaryons; Mushrooms; Nucleotide sequence; Oudemansiella raphanipes; phylogenetic analysis; Phylogeny; Secondary metabolites; Software; Synteny; Taxonomy; China; genome; phylogenetic analysis; Oudemansiella raphanipes; secondary metabolites; CAZyme; monokaryon
• ISSN: 2309-608X; 2309-608X
• DOI: 10.3390/jof9020266

, considered as a well-known culinary edible mushroom with a high content of natural bioactive substances, is widely cultivated in China with the commercial name Changgengu. However, due to the lack of genomic data, molecular and genetic study on is rare. To obtain a comprehensive overview of genetic characteristics and enhance the value of , two mating-compatible monokaryons isolated from the dikaryon were applied for de novo genome sequencing and assembly using Nanopore and /or Illumina sequencing platforms. One of the monokaryons, CGG-A-s1, was annotated with 21,308 protein-coding genes, of which 56 were predicted to be involved in the biosynthesis of secondary metabolites such as terpene, type I PKS, NRPS, and siderophore. Phylogenetic and comparative analysis of multiple fungi genomes revealed a close evolutionary relationship between and based on single-copy orthologous protein genes. Significant collinearity was detected between and on the synteny of inter-species genomes. 664 CAZyme genes in CGG-A-s1 were identified with GHs and AAs families significantly elevated when compared with the other 25 sequenced fungi, indicating a strong wood degradation ability. Furthermore, the mating type locus analysis revealed that CGG-A-s1 and CGG-A-s2 were conserved in the gene organization of the mating locus but various in that of the mating locus. The genome resource of will provide new insights into its development of genetic studies and commercial production of high-quality varieties.