The signal intensity on southern blots developed by nonisotopic methods is linear with time and quantity of DNA

Signal intensity on super(32)P Southern blots is linear with time and DNA concentration. In studying HRAS1 deletions in human breast cancer biopsies with biotinylated probes, it was necessary to establish that colorimetric intensity of allelic bands is linear with time and DNA concentration. The lat...

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Bibliographic Details
Published inNucleic acids research Vol. 17; no. 6; p. 2364
Main Authors LEE, A. S. G, MCGEE, J. Ó'D
Format Journal Article
LanguageEnglish
Published Oxford Oxford University Press 25.03.1989
Subjects
Biological and medical sciences
Biotin
Colorimetry
Diverse techniques
DNA - analysis
DNA Probes
Fundamental and applied biological sciences. Psychology
Kinetics
Molecular and cellular biology
Nucleic Acid Hybridization
Assay
Radiolabelling
DNA
Genetic engineering
Kinetics
Concentration
Technique
Southern blotting
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Summary:Signal intensity on super(32)P Southern blots is linear with time and DNA concentration. In studying HRAS1 deletions in human breast cancer biopsies with biotinylated probes, it was necessary to establish that colorimetric intensity of allelic bands is linear with time and DNA concentration. The latter is demonstrated in this paper in a model system.
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ISSN:0305-1048
1362-4962
DOI:10.1093/nar/17.6.2364