Development of an ELISA for detection of myxoma virus-specific rabbit antibodies: test evaluation for diagnostic applications on vaccinated and wild rabbit sera

An enzyme-linked immunosorbent assay (ELISA) was developed and compared with 2 reference diagnostic tests (indirect immunofluorescence [IF] and complement fixation) to detect myxoma virus-specific antibodies in sera from 50 rabbits experimentally vaccinated with an attenuated strain of myxoma virus...

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Bibliographic Details
Published inJournal of veterinary diagnostic investigation Vol. 11; no. 3; pp. 240 - 245
Main Authors Gelfi, J, Chantal, J, Phong, T.T, Py, R, Boucraut-Baralon, C
Format Journal Article
LanguageEnglish
Published United States 01.05.1999
Subjects
Animal Diseases - diagnosis
Animal Diseases - epidemiology
Animal Diseases - virology
Animals
Antibodies, Viral - analysis
antibody detection
complement fixation tests
disease diagnosis
enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - veterinary
fluorescent antibody technique
Myxoma virus
myxomatosis
Myxomatosis, Infectious - diagnosis
Oryctolagus cuniculus
rabbits
Rabbits - virology
Reference Values
Sensitivity and Specificity
serodiagnosis
Serologic Tests
vaccination
Vaccination - veterinary
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Summary:An enzyme-linked immunosorbent assay (ELISA) was developed and compared with 2 reference diagnostic tests (indirect immunofluorescence [IF] and complement fixation) to detect myxoma virus-specific antibodies in sera from 50 rabbits experimentally vaccinated with an attenuated strain of myxoma virus or with a Shope fibroma virus. The ELISA was highly specific (100% specificity) and sensitive (100%, 21 days after homologous vaccination). In a comparison of the ELISA with the IF test in 128 wild rabbits from France, discrepant results were obtained in only 11 (8.6%) animals, which were positive with the ELISA and negative with the IF test. The higher sensitivity and the good specificity of the ELISA was confirmed in a serologic survey of 118 rabbits from 2 Kerguelen (Indian Ocean) islands, where the prevalence of myxomatosis varied considerably. The ELISA is an alternative serologic test for diagnosis, vaccine evaluation, and seroepidemiologic surveys of myxomatosis.
ISSN:1040-6387
1943-4936
DOI:10.1177/104063879901100306