Hormonal control of the outgrowth of axillary buds in Alstroemeria cultured in vitro

• Published in: Biologia plantarum Vol. 55; no. 4; pp. 664 - 668
• Main Authors: Pumisutapon, P, Visser, R. G. F, De Klerk, G. -J
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01.12.2011; Springer Netherlands; Institute of Experimental Botany of the Czech Academy of Sciences
• Subjects: Alstroemeria; apical dominance; auxin; auxin transport inhibitors; benzyladenine; Biomedical and Life Sciences; buds; cytokinin; fasciation; growth; hormonal regulation; indole butyric acid; inhibition; lanolin; Life Sciences; plant architecture; Plant Sciences; rhizome; shoots; substances; sympodial growth; thidiazuron; sympodial growth; cytokinin; auxin; auxin transport inhibitors; apical dominance; rhizome
• ISSN: 0006-3134; 1573-8264
• DOI: 10.1007/s10535-011-0165-9

We study apical dominance in Alstroemeria, a plant with an architecture very different from the model species used in research on apical dominance. The standard explant was a rhizome with a tip and two vertically growing shoots from which the larger part had been excised leaving ca. 1 cm stem. The axillary buds that resumed growth were located at this 1-cm stem just above the rhizome. They were released by removal of the rhizome tip and the shoot tips. Replacement of excised tips by lanolin with indole-3-butyric acid (IBA) restored apical dominance. The auxin transport inhibitors 2,3,5-triiodobenzoic acid (TIBA) and N-1-napthylphthalamic acid (NPA) reduced apical dominance. 6-Benzylaminopurine (BAP) enhanced axillary bud outgrowth but the highest concentrations (> 9 μM) caused fasciation. Thidiazuron (TDZ) did not show improvement relative to BAP. Even though the architecture of Alstroemeria and the model species are very different, their hormonal mechanisms in apical dominance are for the greater part very similar.