Antioxidant Potential of Sulfated Polysaccharides from Padina boryana ; Protective Effect against Oxidative Stress in In Vitro and In Vivo Zebrafish Model

• Published in: Marine drugs Vol. 18; no. 4; p. 212
• Main Authors: Jayawardena, Thilina U, Wang, Lei, Sanjeewa, K K Asanka, Kang, Sang In, Lee, Jung-Suck, Jeon, You-Jin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 14.04.2020; MDPI
• Subjects: Algae; Animals; antioxidant; Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Apoptosis; Catalase; Damage; Enzymes; Ethanol; Extracellular; Fish; Fourier transforms; Freshwater fishes; Fucoidan; Hydrogen peroxide; Industrial applications; Lipid peroxidation; Lipids; Maldives; Metabolites; Models, Animal; Oxidative stress; Oxidative Stress - drug effects; Padina boryana; Peroxidation; Phaeophyceae; Physiology; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - pharmacology; Pretreatment; Proteins; Reactive oxygen species; Saccharides; Scavenging; Signal transduction; sulfated polysaccharide; Superoxide dismutase; Zebrafish; Maldives; Maldives; antioxidant; sulfated polysaccharide; zebrafish; Padina boryana
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md18040212

Elevated levels of reactive oxygen species (ROS) damage the internal cell components. , a brown alga from the Maldives, was subjected to polysaccharide extraction. The Celluclast enzyme assisted extract (PBE) and ethanol precipitation (PBP) of were assessed against hydrogen peroxide (H O ) induced cell damage and zebra fish models. PBP which contains the majority of sulfated polysaccharides based on fucoidan, showed outstanding extracellular ROS scavenging potential against H O . PBP significantly declined the intracellular ROS levels, and exhibited protection against apoptosis. The study revealed PBPs' ability to activate the Nrf2/Keap1 signaling pathway, consequently initiating downstream elements such that catalase (CAT) and superoxide dismutase (SOD). Further, ROS levels, lipid peroxidation values in zebrafish studies were declined with the pre-treatment of PBP. Collectively, the results obtained in the study suggest the polysaccharides from might be a potent source of water soluble natural antioxidants that could be sustainably utilized in the industrial applications.