Novel Antioxidant Peptides from Crassostrea Hongkongensis Improve Photo-Oxidation in UV-Induced HaCaT Cells

• Published in: Marine drugs Vol. 20; no. 2; p. 100
• Main Authors: Zhang, Chen, Lv, Jiatong, Qin, Xiaoming, Peng, Zhilan, Lin, Haisheng
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 24.01.2022; MDPI
• Subjects: Aging; Animals; Antioxidants; Antioxidants - isolation & purification; Antioxidants - pharmacology; Cells; Chromatography, Liquid; Collagen; Crassostrea - metabolism; Cytotoxicity; Enzymatic activity; Enzyme activity; Fractions; HaCaT Cells; High-performance liquid chromatography; HPLC; Humans; Hydrolysates; Identification; Interstitial collagenase; Liquid chromatography; Marine molluscs; Mass spectrometry; Mass spectroscopy; Matrix metalloproteinase; Metalloproteinase; Molecular docking; Molecular Docking Simulation; Molecular weight; Oxidation; Oxidation-Reduction; Oxidative stress; oyster; Oysters; Peptides; Peptides - isolation & purification; Peptides - pharmacology; photo-oxidation; Photooxidation; Proteins; Quality of life; Senescence; Skin; Skin - drug effects; Skin - radiation effects; Skin Aging - drug effects; Skin cancer; Tandem Mass Spectrometry; Toxicity; Ultrafiltration; Ultraviolet radiation; Ultraviolet Rays; oyster; peptides; HaCaT cells; photo-oxidation
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md20020100

Enzymatic hydrolysates from Oysters (OAH) display multiple biological activities. Previously, a 3~5 KDa oyster ultrafiltration component (OUP) showed a high property of preventing skin oxidation. Subsequently, we identified specific peptides with such activity. OUP was fractionated stepwise by Sephadex-G25 and RP-HPLC, and active fractions were screened using UV-irradiated HaCaT cells. The most active fractions (OP5-3) were analyzed by LC-MS/MS and a total of 17 peptides were identified. Results from mass spectrometry showed that OP5-3 consisted of peptides with a molecular weight range of 841.51-1786.92 Da. Six of these peptides were synthesized for validating the activity of resisting skin oxidation in the same cell model. All six peptides showed varying degrees of antioxidant activity, while pretreatment of HaCaT cells with AIVAEVNEAAK alleviated UV cytotoxicity, inhibited metalloproteinase 1 (MMP-1) expression, and showed the highest activity to resist UV-induced skin photo-oxidation among these peptides. In addition, results from molecular docking analysis of MMP-1 with AIVAEVNEAAK showed that AIVAEVNEAAK suppresses its enzymatic activity by directly interacting with MMP-1 and thus exhibit anti-photoaging activity.