Molecular Networking Leveraging the Secondary Metabolomes Space of Halophila stipulaceae (Forsk.) Aschers. and Thalassia hemprichii (Ehrenb. ex Solms) Asch. in Tandem with Their Chemosystematics and Antidiabetic Potentials

• Published in: Marine drugs Vol. 19; no. 5; p. 279
• Main Authors: Hegazi, Nesrine M, Saad, Hamada H, Marzouk, Mona M, Abdel Rahman, Mohamed F, El Bishbishy, Mahitab H, Zayed, Ahmed, Ulber, Roland, Ezzat, Shahira M
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 18.05.2021; MDPI
• Subjects: Acetates; Acetic acid; Animals; Annotations; antidiabetic; Antidiabetics; Aquatic ecosystems; Benzoic acid; Blood Glucose - drug effects; Chromatography; Chromatography, High Pressure Liquid; Diabetes; Diabetes mellitus; Diabetes Mellitus, Experimental - drug therapy; Dyslipidemia; Enhalus; Enzyme Assays; Flavonoids; Glucose; Glucose Transporter Type 2 - metabolism; Glycosides; Halophila; Halophila stipulacea; High performance liquid chromatography; HPLC; Hydrocharitaceae; Hydrocharitaceae - chemistry; Hydrocharitaceae - genetics; Hydrolysis; Hypoglycemic Agents - pharmacology; Hypoglycemic Agents - therapeutic use; Indian Ocean; Insulin - blood; Interspecific; Lipid metabolism; Lipids; Liquid chromatography; Male; Malondialdehyde - metabolism; Mass Spectrometry; Mass spectroscopy; Metabolism; Metabolites; Metabolome; molecular networking; Natural products; Networking; Nitric Oxide - blood; Oxidative stress; Phenolic acids; Phenols; Phylogeny; Phytochemicals; Phytochemicals - analysis; Rats; Rats, Wistar; Scientific imaging; Seagrasses; Steviol; Sulfates; Synergism; Tartaric acid; Taxa; Terpenes; Thalassia hemprichii; Indian Ocean; seagrasses; molecular networking; Halophila stipulacea; Hydrocharitaceae; Thalassia hemprichii; antidiabetic; chemosystematics
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md19050279

The Red Sea is one of the most biodiverse aquatic ecosystems. Notably, seagrasses possess a crucial ecological significance. Among them are the two taxa (Forsk.) Aschers., and (Ehrenb. ex Solms) Asch., which were formally ranked together with the genus Enhalus in three separate families. Nevertheless, they have been recently classified as three subfamilies within Hydrocharitaceae. The interest of this study is to explore their metabolic profiles through ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UPLC-HRMS/MS) analysis in synergism with molecular networking and to assess their chemosystematics relationship. A total of 144 metabolites were annotated, encompassing phenolic acids, flavonoids, terpenoids, and lipids. Furthermore, three new phenolic acids; methoxy benzoic acid- -sulphate ( ), -caffeoyl- -hydroxyl dimethoxy benzoyl tartaric acid ( ), dimethoxy benzoic acid- -sulphate ( ), a new flavanone glycoside; hexahydroxy-monomethoxy flavanone- -glucoside ( ), and a new steviol glycoside; rebaudioside- -acetate ( ) were tentatively described. Additionally, the evaluation of the antidiabetic potential of both taxa displayed an inherited higher activity of in alleviating the oxidative stress and dyslipidemia associated with diabetes. Hence, the current research significantly suggested , , and categorization in three different taxonomic ranks based on their intergeneric and interspecific relationship among them and supported the consideration of seagrasses in natural antidiabetic studies.