Intermittent Hypoxic Exposure with High Dose of Arginine Impact on Circulating Mediators of Tissue Regeneration

• Published in: Nutrients Vol. 12; no. 7; p. 1933
• Main Authors: Zembron-Lacny, Agnieszka, Gramacki, Artur, Wawrzyniak-Gramacka, Edyta, Tylutka, Anna, Hertmanowska, Natalia, Kasperska, Anna, Czuba, Miłosz
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 29.06.2020; MDPI
• Subjects: Altitude; Angiogenesis; Arginine; Arginine - administration & dosage; Athletes; Bioavailability; Body composition; Brain-derived neurotrophic factor; Dietary supplements; Double-Blind Method; Exposure; Gene expression; Growth factors; High density lipoprotein; Humans; Hydrogen peroxide; Hydrogen Peroxide - blood; Hypoxia; Hypoxia - physiopathology; Intercellular Signaling Peptides and Proteins - blood; Kinases; Lipids; Male; Metabolism; muscle damage; Muscles; Myogenesis; Nitric oxide; Nitric Oxide - blood; Olympic games; Oxygen Consumption; Placebos; Regeneration (physiology); Regeneration - drug effects; Repair; Sea level; Tissue engineering; Vascular endothelial growth factor; Wrestling - physiology; nitric oxide; athletes; growth factors; muscle damage; hydrogen peroxide
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu12071933

Intermittent exposure to hypoxia (IHE) increases production of reactive oxygen and nitrogen species which, as signalling molecules, participate in tissue injury-repair-regeneration cascade. The process is also stimulated by arginine whose bioavailability is a limiting factor for NO synthesis. The effects of IHE in combination with arginine (Arg) intake on myogenesis and angiogenesis mediators were examined in a randomized and placebo-controlled trial. Blood samples were collected from 38 elite athletes on the 1st, 7th and 14th days during the training camp. The oral doses of arginine (2 × 6 g/day) and/or IHE using hypoxicator GO2Altitude (IHE and Arg/IHE) were applied. Serum NO and H O concentrations increased significantly and were related to muscle damage (CK activity >900 IU/mL) in IHE and Arg/IHE compared to placebo. The changes in NO and H O elevated the levels of circulating growth factors such as HGF, IHG-1, PDGF , BDNF, VEGF and EPO. Modification of the lipid profile, especially reduced non-HDL, was an additional beneficial effect of hypoxic exposure with arginine intake. Intermittent hypoxic exposure combined with high-dose arginine intake was demonstrated to affect circulating mediators of injury-repair-regeneration. Therefore, a combination of IHE and arginine seems to be a potential therapeutic and non-pharmacological method to modulate the myogenesis and angiogenesis in elite athletes.