Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples

Since mid-2020 there have been complexities and difficulties in the standardisation and administration of nasopharyngeal swabs. Coupled with the variable and/or poor accuracy of lateral flow devices, this has led to increased societal 'testing fatigue' and reduced confidence in test result...

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Published inMethods and protocols Vol. 5; no. 2; p. 25
Main Authors Tarantini, Francesco Saverio, Wu, Siyu, Jenkins, Harry, Tellechea Lopez, Ana, Tomlin, Hannah, Hyde, Ralph, Lis-Slimak, Katarzyna, Thompson, Jamie Louise, Pijuan-Galitó, Sara, Scales, Danielle, Kaneko, Kazuyo, Dey, Jayasree, Park, Emily, Hill, Jack, Lee, I-Ning, Doolan, Lara, Arendt-Tranholm, Asta, Denning, Chris, Seedhouse, Claire, Benest, Andrew V
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 07.03.2022
MDPI
Subjects
Accreditation
Chemical spills
Coronaviruses
COVID-19
Disease control
FDA approval
Heat
Life sciences
Protocol
qPCR
Saliva
Sensitivity analysis
Severe acute respiratory syndrome coronavirus 2
Testing laboratories
United Kingdom > UK
United States > US
COVID-19
saliva
qPCR
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Summary:Since mid-2020 there have been complexities and difficulties in the standardisation and administration of nasopharyngeal swabs. Coupled with the variable and/or poor accuracy of lateral flow devices, this has led to increased societal 'testing fatigue' and reduced confidence in test results. Consequently, asymptomatic individuals have developed reluctance towards repeat testing, which remains the best way to monitor COVID-19 cases in the wider population. On the other hand, saliva-based PCR, a non-invasive, highly sensitive, and accurate test suitable for everyone, is gaining momentum as a straightforward and reliable means of detecting SARS-CoV-2 in symptomatic and asymptomatic individuals. Here, we provide an itemised list of the equipment and reagents involved in the process of sample submission, inactivation and analysis, as well as a detailed description of how each of these steps is performed.
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These authors contributed equally to this work.
ISSN:2409-9279
2409-9279
DOI:10.3390/mps5020025