Size-reduced embryos reveal a gradient scaling-based mechanism for zebrafish somite formation

• Published in: Development (Cambridge) Vol. 145; no. 11; p. dev161257
• Main Authors: Ishimatsu, Kana, Hiscock, Tom W, Collins, Zach M, Sari, Dini Wahyu Kartika, Lischer, Kenny, Richmond, David L, Bessho, Yasumasa, Matsui, Takaaki, Megason, Sean G
• Format: Journal Article
• Language: English
• Published: England The Company of Biologists Ltd 01.06.2018
• Subjects: Animals; Basic Helix-Loop-Helix Transcription Factors - physiology; Body Patterning - genetics; Body Size - physiology; Cleavage Stage, Ovum - physiology; Clock gene; Danio rerio; Developmental stages; Embryos; Fibroblast Growth Factors - metabolism; Gene expression; Gene Expression Regulation, Developmental - genetics; Mesoderm; Models, Theoretical; Morphogenesis - genetics; Organ Size - physiology; Scaling; Segmentation; Somites - embryology; Somitogenesis; Zebrafish - embryology; Zebrafish Proteins - physiology; Somite; Scaling; Zebrafish; Mathematical modeling; Quantitative imaging; Fgf gradient; PSM; Segmentation clock
• ISSN: 0950-1991; 1477-9129
• DOI: 10.1242/dev.161257

Little is known about how the sizes of animal tissues are controlled. A prominent example is somite size, which varies widely both within an individual and across species. Despite intense study of the segmentation clock governing the timing of somite generation, how it relates to somite size is poorly understood. Here, we examine somite scaling and find that somite size at specification scales with the length of the presomitic mesoderm (PSM) despite considerable variation in PSM length across developmental stages and in surgically size-reduced embryos. Measurement of clock period, axis elongation speed and clock gene expression patterns demonstrate that existing models fail to explain scaling. We posit a 'clock and scaled gradient' model, in which somite boundaries are set by a dynamically scaling signaling gradient across the PSM. Our model not only explains existing data, but also makes a unique prediction that we confirm experimentally - the formation of periodic 'echoes' in somite size following perturbation of the size of one somite. Our findings demonstrate that gradient scaling plays a central role in both progression and size control of somitogenesis.