Sodium/calcium exchanger in rat olfactory neurons

• Published in: Neurochemistry international Vol. 30; no. 6; pp. 523 - 531
• Main Authors: Noé, Johannes, Tareilus, Erwin, Boekhoff, Ingrid, Breer, Heinz
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.06.1997; Elsevier
• Subjects: Animals; antiserum; Biological and medical sciences; Blotting, Western; Calcium - metabolism; calcium imaging; Carrier Proteins - metabolism; Cilia - metabolism; Epithelium - metabolism; Fluorescent Dyes; Fundamental and applied biological sciences. Psychology; Homeostasis; Immunohistochemistry; Microscopy, Confocal; olfactory neurons; Olfactory Receptor Neurons - metabolism; Olfactory system and olfaction. Gustatory system and gustation; Rats; Rats, Sprague-Dawley; Sodium-Calcium Exchanger; Vertebrates: nervous system and sense organs; sodium/calcium exchanger; antiserum; calcium imaging; olfactory neurons; Vertebrata; Mammalia; Calcium; Rat; Sodium; Rodentia; Sensory neuron; Ion exchange; Olfactory system
• ISSN: 0197-0186; 1872-9754
• DOI: 10.1016/S0197-0186(96)00090-3

The chemo-electrical transduction process in olfactory neurons is accompanied by a rapid and transient increase in intracellular calcium concentrations. The notion that Na +/Ca 2+ exchanger activities may play a major role in extruding calcium ions out of the cell and maintaining Ca 2+ homeostasis in olfactory receptor cells was assessed by means of laser scanning confocal microscopy in combination with the fluorescent indicators Fluo-3 and Fura-Red. The data indicate that high exchanger acitivity, which was inhibited by amiloride derivatives, is located in the dendritic knob and probably in the olfactory cilia. This result was supported by experiments using specific antiserum raised against retinal Na +/Ca 2+ exchanger protein which labelled an immunoreactive protein of 230 kDa in Western blots from olfactory tissue and strongly stained the ciliary layer of the olfactory epithelium.