Effect of Mouse Oocyte Vitrification on Mitochondrial Membrane Potential and Distribution

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 34; no. 1; pp. 99 - 102
• Main Author: 雷涛 郭娜 谭美华 李豫峰
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.02.2014; Department of Reproductive Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030, China
• Subjects: Animals; Cryopreservation - methods; Cryoprotective Agents - pharmacology; Dimethyl Sulfoxide - pharmacology; DMSO; Ethylene Glycol - pharmacology; Female; Fluorescent Dyes - metabolism; Medicine; Medicine & Public Health; Membrane Potential, Mitochondrial - drug effects; Membrane Potential, Mitochondrial - physiology; Mice; Microscopy, Fluorescence; Mitochondria - drug effects; Mitochondria - metabolism; Oocytes - drug effects; Oocytes - physiology; Temperature; Vitrification; 二甲基亚砜; 冷冻保护剂; 和分布; 对照组; 小鼠卵母细胞; 玻璃化冷冻; 线粒体膜电位; oocytes; mice; vitrification; mitochondria
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-014-1238-8

The effects of mouse oocyte vitrification on mitochondrial membrane potential and distribution were explored in this study. The collected mouse oocytes were randomly divided into vitrification and control groups. Ethylene glycol（EG） and dimethylsulphoxide（DMSO） were used as cryoprotectants in the vitrification group. The mitochondrial function and distribution in the oocytes were examined by using the fluorescent probes, JC-1 and Mito Tracker green. The results showed that the ratio of red to green fluorescence in mouse oocytes was significantly decreased after thawing in the vitrification group as compared with the control group（1.28 vs. 1.70, P0.05）. The percentage of polarized distribution of the mitochondria in oocytes was conspicuously reduced in the vitrification group when compared with the control group（31% vs. 63%, P0.05）. It was suggested that vitrification significantly affects the mitochondrial function and distribution in oocytes and reduces the potential of oocyte fertilization and embryo development.