Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition

• Published in: Marine drugs Vol. 15; no. 9; p. 294
• Main Authors: Liu, Fuyan, Zhang, Xiaofeng, Li, Yuqiu, Chen, Qixin, Liu, Fei, Zhu, Xiqiang, Mei, Li, Song, Xinlei, Liu, Xia, Song, Zhigang, Zhang, Jinhua, Zhang, Wen, Ling, Peixue, Wang, Fengshan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 20.09.2017; MDPI
• Subjects: Animals; anti-inflammatory; Anti-inflammatory agents; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; Cell surface; Cells; Cyclooxygenase-2; Glucans - chemistry; Glucans - pharmacology; Humans; Inflammation; Kinases; Lipopolysaccharides; Macrophages; Macrophages - drug effects; MAP kinase; MAP Kinase Signaling System; MAPK; Marine molluscs; Medical sciences; Medicine, Chinese Traditional; Mytilus; Mytilus coruscus; NF-KappaB Inhibitor alpha; NF-κB; NF-κB protein; Nitric-oxide synthase; Nuclear transport; Phagocytosis; Phosphorylation; Prostaglandin E2; Proteins; Signal Transduction - drug effects; SPR analysis; Surface plasmon resonance; THP-1 Cells - drug effects; THP-1 macrophages; TLR2 protein; TLR4; TLR4 protein; Toll-Like Receptor 4; Toll-like receptors; Translocation; Tumor necrosis factor-α; anti-inflammatory; NF-κB; SPR analysis; MAPK; THP-1 macrophages; TLR4
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md15090294

The hard-shelled mussel ( ) has been used as Chinese traditional medicine for thousands of years; however, to date the ingredients responsible for the various beneficial health outcomes attributed to are still unclear. An α-d-Glucan, called MP-A, was isolated from , and observed to exert anti-inflammatory activity in THP-1 human macrophage cells. Specifically, we showed that MP-A treatment inhibited the production of inflammatory markers, including TNF-α, NO, and PGE2, inducible NOS (iNOS), and cyclooxygenase-2 (COX-2), in LPS-activated THP-1 cells. It was also shown to enhance phagocytosis in the analyzed cells, but to severely inhibit the phosphorylation of mitogen-activated protein kinases (MAPKs) and the nuclear translocation of NF-κB P65. Finally, MP-A was found to exhibit a high binding affinity for the cell surface receptor TLR4, but a low affinity for TLR2 and dectin-1, via surface plasmon resonance (SPR) analysis. The study indicates that MP-A suppresses LPS-induced TNF-α, NO and PEG2 production via TLR4/NF-κB/MAPK pathway inhibition, and suggests that MP-A may be a promising therapeutic candidate for diseases associated with TNF-α, NO, and/or PEG2 overproduction.