Legionella pneumophila prevents proliferation of its natural host Acanthamoeba castellanii
Legionella pneumophila is a ubiquitous, pathogenic, Gram-negative bacterium responsible for legionellosis. Like many other amoeba-resistant microorganisms, L. pneumophila resists host clearance and multiplies inside the cell. Through its Dot/Icm type IV secretion system, the bacterium injects more t...
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Published in | Scientific reports Vol. 6; no. 1; p. 36448 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group UK
02.11.2016
Nature Publishing Group |
Subjects | |
Online Access | Get full text |
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Summary: | Legionella pneumophila
is a ubiquitous, pathogenic, Gram-negative bacterium responsible for legionellosis. Like many other amoeba-resistant microorganisms,
L. pneumophila
resists host clearance and multiplies inside the cell. Through its Dot/Icm type IV secretion system, the bacterium injects more than three hundred effectors that modulate host cell physiology in order to promote its own intracellular replication. Here we report that
L. pneumophila
prevents proliferation of its natural host
Acanthamoeba castellanii
. Infected amoebae could not undergo DNA replication and no cell division was observed. The Dot/Icm secretion system was necessary for
L. pneumophila
to prevent the eukaryotic proliferation. The absence of proliferation was associated with altered amoebal morphology and with a decrease of mRNA transcript levels of CDC2b, a putative regulator of the
A. castellanii
cell cycle. Complementation of
CDC28
-deficient
Saccharomyces cerevisiae
by the
CDC2b
cDNA was sufficient to restore proliferation of
CDC28
-deficient
S. cerevisiae
and suggests for the first time that CDC2b from
A. castellanii
could be functional and a
bona fide
cyclin-dependent kinase. Hence, our results reveal that
L. pneumophila
impairs proliferation of
A. castellanii
and this effect could involve the cell cycle protein CDC2b. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 PMCID: PMC5091012 |
ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/srep36448 |