Industrial-Scale Production of Mycotoxin Binder from the Red Yeast Sporidiobolus pararoseus KM281507

• Published in: Journal of fungi (Basel) Vol. 8; no. 4; p. 353
• Main Authors: Tapingkae, Wanaporn, Srinual, Orranee, Lumsangkul, Chompunut, Doan, Hien Van, Chiang, Hsin-I, Manowattana, Atchara, Boonchuay, Pinpanit, Chaiyaso, Thanongsak
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 30.03.2022; MDPI
• Subjects: 300 L bioreactor; Adsorbents; Adsorption; Aflatoxin B1; Bioreactors; Carotenoids; Deoxynivalenol; Feasibility studies; feed additive; Feed additives; gastrointestinal model; Glucose; Glycerol; Lipids; Mycotoxins; Nutritive value; Ochratoxin A; Sporidiobolus pararoseus; Yeast; Zearalenone; β-Glucan; Thailand; United States > US; Germany; Bangkok Thailand; adsorbents; β-glucan; 300 L bioreactor; feed additive; gastrointestinal model
• ISSN: 2309-608X; 2309-608X
• DOI: 10.3390/jof8040353

Red yeast Sporidiobolus pararoseus KM281507 has been recognized as a potential feed additive. Beyond their nutritional value (carotenoids and lipids), red yeast cells (RYCs) containing high levels of β-glucan can bind mycotoxins. This study investigated the industrial feasibility of the large-scale production of RYCs, along with their ability to act as a mycotoxin binder. Under a semi-controlled pH condition in a 300 L bioreactor, 28.70-g/L biomass, 8.67-g/L lipids, and 96.10-mg/L total carotenoids were obtained, and the RYCs were found to contain 5.73% (w/w) β-glucan. The encapsulated RYC was in vitro tested for its mycotoxin adsorption capacity, including for aflatoxin B1 (AFB1), zearalenone (ZEA), ochratoxin A (OTA), T-2 toxin (T-2) and deoxynivalenol (DON). The RYCs had the highest binding capacity for OTA and T-2 at concentrations of 0.31–1.25 and 0.31–2.5 µg/mL, respectively. The mycotoxin adsorption capacity was further tested using a gastrointestinal poultry model. The adsorption capacities of the RYCs and a commercial mycotoxin binder (CMB) were comparable. The RYCs not only are rich in lipids and carotenoids but also play an important role in mycotoxin binding. Since the industrial-scale production and downstream processing of RYCs were successfully demonstrated, RYCs could be applied as possible feed additives.