Role of EctR as transcriptional regulator of ectoine biosynthesis genes in Methylophaga thalassica

• Published in: Biochemistry (Moscow) Vol. 77; no. 8; pp. 857 - 863
• Main Authors: Mustakhimov, I. I., Reshetnikov, A. S., Fedorov, D. N., Khmelenina, V. N., Trotsenko, Y. A.
• Format: Journal Article
• Language: English
• Published: Dordrecht SP MAIK Nauka/Interperiodica 01.08.2012; Springer; Springer Nature B.V
• Subjects: Amino Acids, Diamino - biosynthesis; Bacteria; Bacterial proteins; Biochemistry; Biomedical and Life Sciences; Biomedicine; Bioorganic Chemistry; Biosynthesis; E coli; Enzymes; Escherichia coli; Gene expression; Genes; Genetic aspects; Genetic transcription; Life Sciences; Methylophaga thalassica; Microbiology; Physiological aspects; Piscirickettsiaceae - genetics; Piscirickettsiaceae - metabolism; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Transcription Factors - genetics; Transcription Factors - metabolism; methylotrophic bacterium, osmoprotectant, ectoine genes, transcriptional regulation
• ISSN: 0006-2979; 1608-3040
• DOI: 10.1134/S0006297912080068

In the halophilic aerobic methylotrophic bacterium Methylophaga thalassica , the genes encoding the enzymes for biosynthesis of the osmoprotectant ectoine were shown to be located in operon ectABC-ask . Transcription of the ect -operon was started from the two promoters homologous to the σ 70 -dependent promoter of Escherichia coli and regulated by protein EctR, whose encoding gene, ectR , is transcribed from three promoters. Genes homologous to ectR of methylotrophs were found in clusters of ectoine biosynthesis genes in some non-methylotrophic halophilic bacteria. EctR proteins of methylotrophic and heterotrophic halophiles belong to the MarR-family of transcriptional regulators but form a separate branch on the phylogenetic tree of the MarR proteins.