Temporal Imaging of Live Cells by High-Speed Confocal Raman Microscopy

Label-free live cell imaging was performed using a custom-built high-speed confocal Raman microscopy system. For various cell types, cell-intrinsic Raman bands were monitored. The high-resolution temporal Raman images clearly delineated the intracellular distribution of biologically important molecu...

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Bibliographic Details
Published inMaterials Vol. 14; no. 13; p. 3732
Main Authors Kang, Jeon Woong, Nguyen, Freddy T, Lue, Niyom
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 03.07.2021
MDPI
Subjects
Cameras
cell analysis
Colorectal cancer
High speed
Image reconstruction
Image resolution
Lasers
Lipids
Microscopes
Microscopy
Perturbation
Proteins
Raman microscopy
Raman spectroscopy
Spectrum analysis
United States > US
Japan
Raman spectroscopy
cell analysis
Raman microscopy
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Summary:Label-free live cell imaging was performed using a custom-built high-speed confocal Raman microscopy system. For various cell types, cell-intrinsic Raman bands were monitored. The high-resolution temporal Raman images clearly delineated the intracellular distribution of biologically important molecules such as protein, lipid, and DNA. Furthermore, optical phase delay measured using quantitative phase microscopy shows similarity with the image reconstructed from the protein Raman peak. This reported work demonstrates that Raman imaging is a powerful label-free technique for studying various biomedical problems in vitro with minimal sample preparation and external perturbation to the cellular system.
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ISSN:1996-1944
1996-1944
DOI:10.3390/ma14133732