Functional role of carbohydrate residues in human immunoglobulin G and therapeutic monoclonal antibodies

• Published in: Biochemistry Vol. 81; no. 8; pp. 835 - 857
• Main Authors: Dorokhov, Y. L., Sheshukova, E. V., Kosobokova, E. N., Shindyapina, A. V., Kosorukov, V. S., Komarova, T. V.
• Format: Journal Article Book Review
• Language: English
• Published: Moscow Pleiades Publishing 01.08.2016; Springer; Springer Nature B.V
• Subjects: Animals; Antibodies, Monoclonal - chemistry; Antibodies, Monoclonal - metabolism; Antibodies, Monoclonal - therapeutic use; Biochemistry; Biomedical and Life Sciences; Biomedicine; Bioorganic Chemistry; Carbohydrates; Cytotoxicity; Glycosylation; Health aspects; Humans; Immunoglobulin Fc Fragments - chemistry; Immunoglobulin Fc Fragments - metabolism; Immunoglobulin Fc Fragments - therapeutic use; Immunoglobulin G - chemistry; Immunoglobulin G - metabolism; Immunoglobulin G - therapeutic use; Immunoglobulins; Immunotherapy; Life Sciences; Microbiology; Monoclonal antibodies; Polysaccharides - chemistry; Polysaccharides - metabolism; Polysaccharides - therapeutic use; Review; immunoglobulin G; immunotherapy; glycosylation; monoclonal antibody; antibody-dependent cell cytotoxicity; Chinese hamster ovary cells; biosimilarity
• ISSN: 0006-2979; 1608-3040
• DOI: 10.1134/S0006297916080058

Therapeutic monoclonal antibodies (TMA) provide an important means for treating diseases that were previously considered untreatable. Currently more than 40 full-size TMAs created primarily based on immunoglobulin G1 are widely used for treating various illnesses. Glycosylation of TMA is among other numerous factors that affect their biological activity, effector functions, immunogenicity, and half-life in the patient’s serum. The importance of carbohydrate residues for activity of human serum immunoglobulin and TMA produced in animal cells is considered in this review, with emphasis given to N-glycosylation of the Fc fragment of the antibody.