Clone-specific MYD88 L265P and CXCR4 mutation status can provide clinical utility in suspected Waldenstrom macroglobulinemia/lymphoplasmacytic lymphoma

• Published in: Leukemia research Vol. 51; pp. 41 - 48
• Main Authors: Burnworth, Bettina, PhD, Wang, Zhixing, PhD, Singleton, Timothy P., MD, Bennington, Angela, BS, Fritschle, Wayne, BS, Bennington, Richard, MS, Brodersen, Lisa Eidenschink, PhD, Wells, Denise A., MD, Loken, Michael R., PhD, Zehentner, Barbara K., Dr
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.12.2016
• Subjects: Aged; Aged, 80 and over; B-Lymphocytes - chemistry; B-Lymphocytes - pathology; Biomarkers, Tumor - analysis; Clone Cells; CXCR4; Female; Flow cytometric cell sorting; Hematology, Oncology and Palliative Medicine; Humans; Lymphoplasmacytic lymphoma; Male; Middle Aged; Mutation; MYD88L256P; Myeloid Differentiation Factor 88 - analysis; Myeloid Differentiation Factor 88 - genetics; Plasma Cells - chemistry; Plasma Cells - pathology; Polymerase Chain Reaction; Receptors, CXCR4 - analysis; Receptors, CXCR4 - genetics; Sensitivity and Specificity; Tumor Cells, Cultured; Waldenstrom Macroglobulinemia - diagnosis; Waldenstrom Macroglobulinemia - pathology; Waldenström’s macroglobulinemia; CXCR4; flow cytometric cell sorting; MYD88L256P; lymphoplasmacytic lymphoma; Waldenström’s Macroglobulinemia; Lymphoplasmacytic lymphoma; Flow cytometric cell sorting; Waldenström’s macroglobulinemia
• ISSN: 0145-2126; 1873-5835
• DOI: 10.1016/j.leukres.2016.10.008

Highlights • Sorted B- and plasma cells are clonally unrelated in the majority of suspected LPL. • MYD88 L265P is found in both fractions for confirmed WM, but not for suspected LPL. • Specificity of MYD88 L265P is enhanced when detected in both B- and plasma cells. • Sensitivity for CXCR4 analysis is increased by flow cytometric cell sorting. • Magnetic beads cannot replace flow cytometric cell sorting for molecular analysis.