Effect of cyclic AMP on ciliary activity of human respiratory epithelium

In order to investigate the effect of cyclic adenosine monophosphate (cAMP) on ciliary beat frequency (CBF) in human respiratory epithelium, cells were brushed from the inferior nasal turbinates of three groups of ten subjects: awake adults (aged 20-34 yrs), anaesthetized children (2-15 yrs) and ana...

Full description

Saved in:
Bibliographic Details
Published inThe European respiratory journal Vol. 4; no. 7; pp. 789 - 795
Main Authors Di Benedetto, G, Manara-Shediac, FS, Mehta, A
Format Journal Article
LanguageEnglish
Published Leeds Eur Respiratory Soc 01.07.1991
Maney
Subjects
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Adolescent
Adult
Age Factors
Anesthesia, General
Biological and medical sciences
Bucladesine - pharmacology
Child
Child, Preschool
Cilia - metabolism
Epithelial Cells
Epithelium - metabolism
Female
Humans
In Vitro Techniques
Isoquinolines - pharmacology
Male
Medical sciences
Nasal Mucosa - cytology
Nasal Mucosa - metabolism
Piperazines - pharmacology
Pneumology
Protein Kinase Inhibitors
Human
Treatment
Respiratory disease
Lung
Adult
Ciliary activity
Epithelium
Result
Online AccessGet full text

Cover

More Information
Summary:In order to investigate the effect of cyclic adenosine monophosphate (cAMP) on ciliary beat frequency (CBF) in human respiratory epithelium, cells were brushed from the inferior nasal turbinates of three groups of ten subjects: awake adults (aged 20-34 yrs), anaesthetized children (2-15 yrs) and anaesthetized adults (19-61 yrs). Cells from the awake adults were also studied after storage for 24 h in tissue culture medium. CBF was measured in vitro with a photometric technique at room temperature (22.0 +/- 1.5 degrees C). Samples were mounted in a perfusion chamber and challenged with either control solutions, dibutyryl cAMP (10(-4) or 10(-3) M), or the cyclic nucleotide-dependent protein kinase inhibitor 1-(5-isoquinolinylsulphonyl)-2-methylpiperazine (H-7). Dibutyryl cAMP (10(-3) M) caused a significant increase in CBF in all groups studied: awake adults (+1.1 Hz; p less than 0.01), anaesthetized children (+1.2 Hz; p less than 0.01), anaesthetized adults (+1.2 Hz; p less than 0.01), stored cells (+1.1 Hz; p less than 0.01). This response was inhibited by preincubation with H-7 (10(-4) and 10(-3) M). It is concluded that cAMP is a regulator of ciliary activity in human respiratory epithelium.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0903-1936
1399-3003
DOI:10.1183/09031936.93.04070789