Influence of Dietary Lipids and Environmental Salinity on the n-3 Long-Chain Polyunsaturated Fatty Acids Biosynthesis Capacity of the Marine Teleost Solea senegalensis

• Published in: Marine drugs Vol. 19; no. 5; p. 254
• Main Authors: Marrero, Manuel, Monroig, Óscar, Betancor, Mónica, Herrera, Marcelino, Pérez, José A, Garrido, Diego, Galindo, Ana, Giráldez, Inmaculada, Rodríguez, Covadonga
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 29.04.2021; MDPI
• Subjects: Aquaculture; Biosynthesis; Commercial fishing; Composition; Desaturase; Diet; Docosahexaenoic acid; Eicosapentaenoic acid; Elovl5; Enterocytes; Enzymatic activity; Enzyme activity; Enzymes; Fads2; Fatty acid composition; Fatty acids; Fish; Fish oils; Fisheries; Hepatocytes; Incubation period; Intestine; Intestines; LC-PUFA biosynthesis; Linolenic acid; Lipids; Marine fishes; Muscles; Polyunsaturated fatty acids; Salinity; Salinity effects; Seafood; Solea senegalensis; Vegetable oils; Fads2; LC-PUFA biosynthesis; salinity; Elovl5; EPA; diet; DHA; Solea senegalensis
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md19050254

Fish vary in their ability to biosynthesise long-chain polyunsaturated fatty acids (LC-PUFA) depending upon the complement and function of key enzymes commonly known as fatty acyl desaturases and elongases. It has been reported in the existence of a Δ4 desaturase, enabling the biosynthesis of docosahexaenoic acid (DHA) from eicosapentaenoic acid (EPA), which can be modulated by the diet. The present study aims to evaluate the combined effects of the partial replacement of fish oil (FO) with vegetable oils and reduced environmental salinity in the fatty acid composition of relevant body compartments (muscle, hepatocytes and enterocytes), the enzymatic activity over α-linolenic acid (ALA) to form n-3 LC-PUFA through the incubation of isolated hepatocytes and enterocytes with [1- C] 18:3 n-3, and the regulation of the and in the liver and intestine. The presence of radiolabelled products, including 18:4n-3, 20:4n-3 and EPA, provided compelling evidence that a complete pathway enabling the biosynthesis of EPA from ALA, establishing , has at least one Fads2 with ∆6 activity. Dietary composition prevailed over salinity in regulating the expression of , while salinity did so over dietary composition for . FO replacement enhanced the proportion of DHA in muscle and the combination with 20 ppt salinity increased the amount of n-3 LC-PUFA in hepatocytes.