Short chain N-acyl homoserine lactone production by soil isolate Burkholderia sp. strain A9

• Published in: Sensors (Basel, Switzerland) Vol. 13; no. 10; pp. 13217 - 13227
• Main Authors: Chen, Jian Woon, Koh, Chong-Lek, Sam, Choon-Kook, Yin, Wai-Fong, Chan, Kok-Gan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 30.09.2013; Molecular Diversity Preservation International (MDPI)
• Subjects: 4-Butyrolactone - analogs & derivatives; 4-Butyrolactone - biosynthesis; 4-Butyrolactone - isolation & purification; Acids; Antibiotics; Bacteria; Biosensing Techniques - methods; Burkholderia - classification; Burkholderia - isolation & purification; Burkholderia - metabolism; CepI/CepR homologs; Chromatography; E coli; Lasers; MALDI-TOF mass spectrometry; Mass spectrometry; Microorganisms; N-hexanoylhomoserine lactone; Quorum Sensing - physiology; Science education; Scientific imaging; Sensors; Soil Microbiology; Species Specificity; triple quadrupole LC/MS; tropical soil bacteria; Virulence; Malaysia; Germany; Singapore
• ISSN: 1424-8220; 1424-8220
• DOI: 10.3390/s131013217

In the bacteria kingdom, quorum sensing (QS) is a cell-to-cell communication that relies on the production of and response to specific signaling molecules. In proteobacteria, N-acylhomoserine lactones (AHLs) are the well-studied signaling molecules. The present study aimed to characterize the production of AHL of a bacterial strain A9 isolated from a Malaysian tropical soil. Strain A9 was identified as Burkholderia sp. using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and 16S rDNA nucleotide sequence analysis. AHL production by A9 was detected with two biosensors, namely Chromobacterium violaceum CV026 and Escherichia coli [pSB401]. Thin layer chromatography results showed N-hexanoylhomoserine lactone (C6-HSL) and N-octanoylhomoserine lactone (C8-HSL) production. Unequivocal identification of C6-HSL and C8-HSL was achieved by high resolution triple quadrupole liquid chromatography-mass spectrometry analysis. We have demonstrated that Burkholderia sp. strain A9 produces AHLs that are known to be produced by other Burkholderia spp. with CepI/CepR homologs.