Fucoidan-Stabilized Gold Nanoparticle-Mediated Biofilm Inhibition, Attenuation of Virulence and Motility Properties in Pseudomonas aeruginosa PAO1

• Published in: Marine drugs Vol. 17; no. 4; p. 208
• Main Authors: Khan, Fazlurrahman, Manivasagan, Panchanathan, Lee, Jang-Won, Pham, Dung Thuy Nguyen, Oh, Junghwan, Kim, Young-Mog
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 03.04.2019; MDPI
• Subjects: Algae; Analytical methods; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial activity; antibiofilm; Antibiotic resistance; Antibiotics; Attenuation; Bacteria; Biofilms; Biofilms - drug effects; Cell culture; Disease resistance; Drug Compounding - methods; Drug resistance; Drug Resistance, Bacterial - drug effects; Drug Stability; Electron microscopy; Field emission microscopy; Field emission spectroscopy; Fourier transforms; Fucoidan; Gold; Gold - chemistry; Gold - pharmacology; Infrared spectroscopy; Laboratories; Light scattering; Metal Nanoparticles - chemistry; Microbial Sensitivity Tests; Minimum inhibitory concentration; Morphology; motility; Nanoparticles; Opportunist infection; Pathogens; Phaeophyceae - chemistry; Photon correlation spectroscopy; Polysaccharides - chemistry; Polysaccharides - pharmacology; Pseudomonas aeruginosa; Pseudomonas aeruginosa - drug effects; Pseudomonas aeruginosa - physiology; Pseudomonas Infections - drug therapy; Pseudomonas Infections - microbiology; Scanning electron microscopy; Seaweed - chemistry; Seaweeds; Spectrum analysis; Swarming; Swimming; Transmission electron microscopy; Twitching; Virulence; Virulence factors; X-ray diffraction; United States > US; Japan; Pseudomonas aeruginosa; motility; nanoparticles; antibiofilm; fucoidan; virulence factors
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md17040208

The emergence of antibiotic resistance in due to biofilm formation has transformed this opportunistic pathogen into a life-threatening one. Biosynthesized nanoparticles are increasingly being recognized as an effective anti-biofilm strategy to counter biofilms. In the present study, gold nanoparticles (AuNPs) were biologically synthesized and stabilized using fucoidan, which is an active compound sourced from brown seaweed. Biosynthesized fucoidan-stabilized AuNPs (F-AuNPs) were subjected to characterization using UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), field emission transmission electron microscopy (FE-TEM), dynamic light scattering (DLS), and energy dispersive X-ray diffraction (EDX). The biosynthesized F-AuNPs were then evaluated for their inhibitory effects on bacterial growth, biofilm formation, virulence factor production, and bacterial motility. Overall, the activities of F-AuNPs towards were varied depending on their concentration. At minimum inhibitory concentration (MIC) (512 µg/mL) and at concentrations above MIC, F-AuNPs exerted antibacterial activity. In contrast, the sub-inhibitory concentration (sub-MIC) levels of F-AuNPs inhibited biofilm formation without affecting bacterial growth, and eradicated matured biofilm. The minimum biofilm inhibition concentration (MBIC) and minimum biofilm eradication concentration (MBEC) were identified as 128 µg/mL. Furthermore, sub-MICs of F-AuNPs also attenuated the production of several important virulence factors and impaired bacterial swarming, swimming, and twitching motilities. Findings from the present study provide important insights into the potential of F-AuNPs as an effective new drug for controlling -biofilm-related infections.