Phenotyping soybean plants transformed with rd29A:AtDREB1A for drought tolerance in the greenhouse and field

• Published in: Transgenic research Vol. 23; no. 1; pp. 75 - 87
• Main Authors: de Paiva Rolla, Amanda Alves, de Fátima Corrêa Carvalho, Josirley, Fuganti-Pagliarini, Renata, Engels, Cibelle, do Rio, Alexandre, Marin, Silvana Regina Rockenbach, de Oliveira, Maria Cristina Neves, Beneventi, Magda A, Marcelino-Guimarães, Francismar Corrêa, Farias, José Renato Bouças, Neumaier, Norman, Nakashima, Kazuo, Yamaguchi-Shinozaki, Kazuko, Nepomuceno, Alexandre Lima
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01.02.2014; Springer Netherlands; Springer Nature B.V
• Subjects: Adaptation, Physiological - genetics; Animal Genetics and Genomics; Arabidopsis - genetics; Arabidopsis Proteins - genetics; asexual reproduction; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; drought; drought tolerance; Droughts; Gene Expression Regulation, Plant; genes; Genetic Engineering; Glycine max - genetics; Glycine max - growth & development; irrigation; Life Sciences; Molecular Medicine; Original Paper; Plant Genetics and Genomics; Plants, Genetically Modified; pods; seeds; Seeds - genetics; Seeds - growth & development; soil; soybeans; transcription factors; Transcription Factors - genetics; Transgenics; transpiration; vegetative growth; Water - metabolism; water stress; yield components; Transpiration; BR 16; Transcription factor; Water deficit; Embrapa 48
• ISSN: 0962-8819; 1573-9368
• DOI: 10.1007/s11248-013-9723-6

The development of drought tolerant plants is a high priority because the area suffering from drought is expected to increase in the future due to global warming. One strategy for the development of drought tolerance is to genetically engineer plants with transcription factors (TFs) that regulate the expression of several genes related to abiotic stress defense responses. This work assessed the performance of soybean plants overexpressing the TF DREB1A under drought conditions in the field and in the greenhouse. Drought was simulated in the greenhouse by progressively drying the soil of pot cultures of the P58 and P1142 lines. In the field, the performance of the P58 line and of 09D-0077, a cross between the cultivars BR16 and P58, was evaluated under four different water regimes: irrigation, natural drought (no irrigation) and water stress created using rain-out shelters in the vegetative or reproductive stages. Although the dehydration-responsive element-binding protein (DREB) plants did not outperform the cultivar BR16 in terms of yield, some yield components were increased when drought was introduced during the vegetative stage, such as the number of seeds, the number of pods with seeds and the total number of pods. The greenhouse data suggest that the higher survival rates of DREB plants are because of lower water use due to lower transpiration rates under well watered conditions. Further studies are needed to better characterize the soil and atmospheric conditions under which these plants may outperform the non-transformed parental plants.