Rapid preparation of functional polysaccharides from Pyropia yezoensis by microwave-assistant rapid enzyme digest system

• Published in: Carbohydrate polymers Vol. 153; pp. 512 - 517
• Main Authors: Lee, Ji-Hyeok, Kim, Hyung-Ho, Ko, Ju-Young, Jang, Jun-Ho, Kim, Gwang-Hoon, Lee, Jung-Suck, Nah, Jae-Woon, Jeon, You-Jin
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 20.11.2016
• Subjects: Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Antioxidative effect; Chemical Fractionation; Low molecular weight polysaccharides; Microwave-assisted rapid enzyme digest system; Microwaves; Monosaccharides - chemistry; Monosaccharides - isolation & purification; Monosaccharides - pharmacology; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Pyropia yezoensis; Rhodophyta - chemistry; Ultrafiltration; Antioxidative effect; Low molecular weight polysaccharides; Pyropia yezoensis; Microwave-assisted rapid enzyme digest system
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2016.07.122

•Pyropia yezoensis is rich in functional sulfated polysaccharides.•Harmful acids were used in the typical polysaccharide extraction or isolation.•Microwave-assistant enzyme digest system helps a rapid isolation of the polysaccharides.•The isolated polysaccharide has strong antioxidant effect. This study describes a simple preparation of functional polysaccharides from Pyropia yezoensis using a microwave-assistant rapid enzyme digest system (MAREDS) with various carbohydrases, and evaluates their antioxidative effects. Polysaccharide hydrolysates were prepared using MAREDS under different hydrolytic conditions of the carbohydrases and microwave powers. Polysaccharides less than 10kDa (Low molecular weight polysaccharides, LMWP, ≤10kDa) were efficiently obtained using an ultrafiltration (molecular weight cut–off of 10kDa). MAREDS increases AMG activation via an increased degree of hydrolysis; the best AMG hydrolysate was prepared using a 10:1 ratio of substrate to enzyme for 2h in MAREDS with 400W. LMWP consisted of galactose (27.3%), glucose (64.5%), and mannose (8.3%) from the AMG hydrolysate had stronger antioxidant effects than the high molecular weight polysaccharides (>10kDa). We rapidly prepared functional LMWPs by using MAREDS with carbohydrases, and suggest that LMWP might be potentially a valuable algal polysaccharide antioxidant.