Anti-Vascular Inflammatory Effect of Ethanol Extract from Securinega suffruticosa in Human Umbilical Vein Endothelial Cells

• Published in: Nutrients Vol. 12; no. 11; p. 3448
• Main Authors: Han, Byung Hyuk, Song, Chun Ho, Yoon, Jung Joo, Kim, Hye Yoom, Seo, Chang Seob, Kang, Dae Gill, Lee, Yun Jung, Lee, Ho Sub
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 10.11.2020; MDPI
• Subjects: Adhesion; adhesion molecules; AKT protein; Anti-Inflammatory Agents - pharmacology; Arteriosclerosis; Atherosclerosis; Blood circulation; Cell adhesion; Cell adhesion & migration; Cell adhesion molecules; Chemical compounds; Cytokines; Cytoplasm; Endothelial cells; Endothelium; Ethanol; Ethanol - isolation & purification; Human Umbilical Vein Endothelial Cells - cytology; Human Umbilical Vein Endothelial Cells - drug effects; Human Umbilical Vein Endothelial Cells - metabolism; Humans; HUVECs; Inflammation; Inflammation - metabolism; Inflammation - prevention & control; Intercellular adhesion molecule 1; Intercellular Adhesion Molecule-1 - genetics; Intercellular Adhesion Molecule-1 - metabolism; Interleukin 6; Intracellular; Kinases; Leaves; Life sciences; Lipoproteins, LDL; Low density lipoprotein; mRNA; Muscles; NF-kappa B - metabolism; NF-κB protein; Nitric oxide; Nitric Oxide Synthase Type III - metabolism; Nitric-oxide synthase; Oxidative stress; Pharmacology; Phosphorylation; Plant Extracts - chemistry; Plant Extracts - pharmacology; Pretreatment; Proteins; Proto-Oncogene Proteins c-akt - metabolism; Reactive Oxygen Species - metabolism; Securinega - chemistry; Securiniga suffruticosa; Spleen; Tendons; TNF-α; Translocation; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Umbilical vein; Vascular cell adhesion molecule 1; Vascular Cell Adhesion Molecule-1 - genetics; Vascular Cell Adhesion Molecule-1 - metabolism; vascular inflammation; Veins; Veins & arteries; United States > US; China; Japan; Germany; NO; vascular inflammation; Securiniga suffruticosa; TNF-α; adhesion molecules; HUVECs
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu12113448

is known as a drug that has the effect of improving the blood circulation and relaxing muscles and tendons, thereby protects and strengthen kidney and spleen. Therefore, in this study, treatment of showed protective effect of inhibiting the vascular inflammation in human umbilical vein endothelial cells (HUVECs) by inducing nitric oxide (NO) production and endothelial nitric oxide synthase (eNOS) coupling pathway. In this study, suppressed TNF-α (Tumor necrosis factor-α) induced protein and mRNA levels of cell adhesion molecules such as intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and Interleukin-6 (IL-6). Pretreatment of HUVEC with decreased the adhesion of HL-60 cells to Ox-LDL (Oxidized Low-Density-Lipoprotein)-induced HUVEC. Moreover, inhibited TNF-α induced intracellular reactive oxygen species (ROS) production. also inhibited phosphorylation of IκB-α in cytoplasm and translocation of NF-κB (Nuclear factor-kappa B) p65 to the nucleus. increased NO production, as well increased the phosphorylation of eNOS and Akt (protein kinase B) which are related with NO production. In addition, increased the protein expression of GTPCH (Guanosine triphosphate cyclohydrolase Ⅰ) and the production of BH4 in HUVEC which are related with eNOS coupling pathway. In conclusion, has a protective effect against vascular inflammation and can be a potential therapeutic agent for early atherosclerosis.