In Vitro Antioxidant Activities of Enzymatic Hydrolysate from Schizochytrium sp. and Its Hepatoprotective Effects on Acute Alcohol-Induced Liver Injury In Vivo

• Published in: Marine drugs Vol. 15; no. 4; p. 115
• Main Authors: Cai, Xixi, Yan, Ana, Fu, Nanyan, Wang, Shaoyun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 10.04.2017; MDPI
• Subjects: Alanine; Alanine transaminase; Alanine Transaminase - metabolism; Alcohol; alcohol-induced liver injury; Alcohols; Amino acids; Animals; antioxidant; Antioxidants; Antioxidants - pharmacology; Aspartate aminotransferase; Aspartate Aminotransferases - metabolism; Carbon Tetrachloride - pharmacology; Catalase; Catalase - metabolism; Chemical and Drug Induced Liver Injury - drug therapy; Chemical and Drug Induced Liver Injury - metabolism; Enzymolysis; Ethanol - pharmacology; Exploitation; Fungi - chemistry; Glutathione - metabolism; Glutathione peroxidase; Glutathione Peroxidase - metabolism; hepatoprotective effects; Hydrolysis; Hydrophobicity; Lipid peroxidation; Lipid Peroxidation - drug effects; Lipids; Liver; Liver - drug effects; Liver - metabolism; Male; Malondialdehyde; Malondialdehyde - metabolism; Mice; Molecular weight; Oral administration; Oxidation-Reduction - drug effects; Oxidative stress; Oxidative Stress - drug effects; Peroxidase; Peroxidation; Phytotherapy - methods; Protective Agents - pharmacology; protein hydrolysate; Protein Hydrolysates - pharmacology; Proteins; Schizochytrium; Serum; Subtilisin; Superoxide dismutase; Superoxide Dismutase - metabolism; Ultrafiltration; Weight; Schizochytrium; antioxidant; protein hydrolysate; alcohol-induced liver injury; hepatoprotective effects

protein hydrolysate (SPH) was prepared through stepwise enzymatic hydrolysis by alcalase and flavourzyme sequentially. The proportion of hydrophobic amino acids of SPH was 34.71%. The molecular weight (MW) of SPH was principally concentrated at 180-3000 Da (52.29%). SPH was divided into two fraction...