In Vitro Antioxidant Activities of Enzymatic Hydrolysate from Schizochytrium sp. and Its Hepatoprotective Effects on Acute Alcohol-Induced Liver Injury In Vivo

• Published in: Marine drugs Vol. 15; no. 4; p. 115
• Main Authors: Cai, Xixi, Yan, Ana, Fu, Nanyan, Wang, Shaoyun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 10.04.2017; MDPI
• Subjects: Alanine; Alanine transaminase; Alanine Transaminase - metabolism; Alcohol; alcohol-induced liver injury; Alcohols; Amino acids; Animals; antioxidant; Antioxidants; Antioxidants - pharmacology; Aspartate aminotransferase; Aspartate Aminotransferases - metabolism; Carbon Tetrachloride - pharmacology; Catalase; Catalase - metabolism; Chemical and Drug Induced Liver Injury - drug therapy; Chemical and Drug Induced Liver Injury - metabolism; Enzymolysis; Ethanol - pharmacology; Exploitation; Fungi - chemistry; Glutathione - metabolism; Glutathione peroxidase; Glutathione Peroxidase - metabolism; hepatoprotective effects; Hydrolysis; Hydrophobicity; Lipid peroxidation; Lipid Peroxidation - drug effects; Lipids; Liver; Liver - drug effects; Liver - metabolism; Male; Malondialdehyde; Malondialdehyde - metabolism; Mice; Molecular weight; Oral administration; Oxidation-Reduction - drug effects; Oxidative stress; Oxidative Stress - drug effects; Peroxidase; Peroxidation; Phytotherapy - methods; Protective Agents - pharmacology; protein hydrolysate; Protein Hydrolysates - pharmacology; Proteins; Schizochytrium; Serum; Subtilisin; Superoxide dismutase; Superoxide Dismutase - metabolism; Ultrafiltration; Weight; Schizochytrium; antioxidant; protein hydrolysate; alcohol-induced liver injury; hepatoprotective effects
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md15040115

protein hydrolysate (SPH) was prepared through stepwise enzymatic hydrolysis by alcalase and flavourzyme sequentially. The proportion of hydrophobic amino acids of SPH was 34.71%. The molecular weight (MW) of SPH was principally concentrated at 180-3000 Da (52.29%). SPH was divided into two fractions by ultrafiltration: SPH-I (MW < 3 kDa) and SPH-II (MW > 3 kDa). Besides showing lipid peroxidation inhibitory activity in vitro, SPH-I exhibited high DPPH and ABTS radicals scavenging activities with IC of 350 μg/mL and 17.5 μg/mL, respectively. In addition, the antioxidant activity of SPH-I was estimated in vivo using the model of acute alcohol-induced liver injury in mice. For the hepatoprotective effects, oral administration of SPH-I at different concentrations (100, 300 mg/kg BW) to the mice subjected to alcohol significantly decreased serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and hepatic malondialdehyde (MDA) level compared to the untreated mice. Besides, SPH-I could effectively restore the hepatic superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities and glutathione (GSH) level. Results suggested that SPH was rich in biopeptides that could be exploited as antioxidant molecules against oxidative stress in human body.