Briavioids E-G, Newly Isolated Briarane-Diterpenoids from a Cultured Octocoral Briareum violaceum

• Published in: Marine drugs Vol. 21; no. 2; p. 124
• Main Authors: Huynh, Thanh Hao, Liu, Chia-Jung, Liu, Yi-Hung, Chien, Su-Ying, Wen, Zhi-Hong, Fang, Lee-Shing, Chen, Jih-Jung, Wu, Yang-Chang, Su, Jui-Hsin, Sung, Ping-Jyun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 14.02.2023; MDPI
• Subjects: Absolute configuration; anti-inflammation; Anti-inflammatory agents; briarane; Briareum; Briareum violaceum; briavioid; Carbon; Communication; Cyclooxygenase-2; Diterpenes; Inflammation; iNOS; Invertebrates; Lipopolysaccharides; Macrophages; Metabolites; Natural products; Nitric oxide; Nitric-oxide synthase; Radiation sources; Screening; Single crystals; X-ray; X-ray diffraction; X-ray diffraction analysis; briavioid; COX-2; Briareum violaceum; iNOS; anti-inflammation; X-ray; briarane
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md21020124

The chemical screening of a cultured soft coral, , led to the isolation of eight natural, briarane-related diterpenoids, including three unreported metabolites, briavioids E-G ( - ), and five known briaranes, briacavatolides B ( ) and C ( ), briaexcavatin L ( ), briaexcavatolide U ( ) and briarenol K ( ). The structures of briaranes - were established using spectroscopic methods. The absolute configuration of briavioid A ( ), obtained in a previous study, was reported for the first time in this study by a single-crystal X-ray diffraction analysis using a copper radiation source. The anti-inflammatory activity of briaranes and and briaranes - was evaluated by screening their inhibitory ability against the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins in lipopolysaccharide (LPS)-induced RAW 264.7 macrophage cells.